Protein Info for PP_0722 in Pseudomonas putida KT2440

Annotation: Ribose-phosphate pyrophosphokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 313 PF13793: Pribosyltran_N" amino acids 4 to 121 (118 residues), 173.3 bits, see alignment E=2.2e-55 TIGR01251: ribose-phosphate diphosphokinase" amino acids 4 to 313 (310 residues), 411.6 bits, see alignment E=8.1e-128 PF00156: Pribosyltran" amino acids 158 to 265 (108 residues), 73.7 bits, see alignment E=1.6e-24 PF14572: Pribosyl_synth" amino acids 202 to 313 (112 residues), 120.2 bits, see alignment E=1.6e-38

Best Hits

Swiss-Prot: 100% identical to KPRS_PSEPK: Ribose-phosphate pyrophosphokinase (prs) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00948, ribose-phosphate pyrophosphokinase [EC: 2.7.6.1] (inferred from 100% identity to ppu:PP_0722)

MetaCyc: 66% identical to ribose-phosphate diphosphokinase (Escherichia coli K-12 substr. MG1655)
Ribose-phosphate diphosphokinase. [EC: 2.7.6.1]

Predicted SEED Role

"Ribose-phosphate pyrophosphokinase (EC 2.7.6.1)" in subsystem De Novo Purine Biosynthesis or Pentose phosphate pathway (EC 2.7.6.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.1

Use Curated BLAST to search for 2.7.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PX6 at UniProt or InterPro

Protein Sequence (313 amino acids)

>PP_0722 Ribose-phosphate pyrophosphokinase (Pseudomonas putida KT2440)
MSKMMVFTGNANPDLARRVVRQLHIPLGDVSVGKFSDGEISTEINENVRGKDVFIIQPTC
APTNDNLMELVVMADAFRRSSASRITAVIPYFGYARQDRRPRSARVAISAKVVADMLTVV
GIDRVLTVDLHADQIQGFFDIPVDNIYGSPVLVDDIEDQRFENLMIVSPDIGGVVRARAV
AKSLGVDLGIIDKRREKANHSEVMHIIGDVEGRTCILVDDMVDTAGTLCHAAKALKEHGA
AKVYAYCTHPVLSGRAIENIEKSVLDELVVTNTVPLSAAAQACDRIRQLDIAPVVAEAVR
RISNEESISAMFR