Protein Info for PP_0721 in Pseudomonas putida KT2440

Annotation: 50S ribosomal protein L25

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 217 signal peptide" amino acids 1 to 14 (14 residues), see Phobius details PF01386: Ribosomal_L25p" amino acids 26 to 113 (88 residues), 91.8 bits, see alignment E=3.1e-30 TIGR00731: ribosomal protein bL25, Ctc-form" amino acids 26 to 202 (177 residues), 163 bits, see alignment E=2.8e-52 PF14693: Ribosomal_TL5_C" amino acids 121 to 207 (87 residues), 92.6 bits, see alignment E=1.6e-30

Best Hits

Swiss-Prot: 100% identical to RL25_PSEPK: 50S ribosomal protein L25 (rplY) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K02897, large subunit ribosomal protein L25 (inferred from 100% identity to ppu:PP_0721)

Predicted SEED Role

"LSU ribosomal protein L25p" in subsystem Ribosome LSU bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PX7 at UniProt or InterPro

Protein Sequence (217 amino acids)

>PP_0721 50S ribosomal protein L25 (Pseudomonas putida KT2440)
MPSPLALVANALGGLLWRNKMTEFTLNAQARTDLGKGASRRLRHSANIPAVVYGGNQDAQ
SLTILAKEITKLFENEAAFSHVLELNVDGAKQNVVVKALQRHPAKGFIMHADFVRVVAGQ
KLTAKVPVHFINEEAPVKKGGEISHVESEIEVSCEAKDLPEFIEVDLANAEIGTIIHLSD
LKAPKGVEFVALAHGDDKAVANVHAPRVAPEAEGAAE