Protein Info for PP_0709 in Pseudomonas putida KT2440

Annotation: Transporter, NCS1 nucleoside transporter family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 499 transmembrane" amino acids 28 to 50 (23 residues), see Phobius details amino acids 57 to 80 (24 residues), see Phobius details amino acids 100 to 120 (21 residues), see Phobius details amino acids 141 to 163 (23 residues), see Phobius details amino acids 170 to 190 (21 residues), see Phobius details amino acids 204 to 227 (24 residues), see Phobius details amino acids 243 to 269 (27 residues), see Phobius details amino acids 291 to 315 (25 residues), see Phobius details amino acids 327 to 349 (23 residues), see Phobius details amino acids 358 to 381 (24 residues), see Phobius details amino acids 416 to 436 (21 residues), see Phobius details amino acids 448 to 468 (21 residues), see Phobius details PF02133: Transp_cyt_pur" amino acids 14 to 436 (423 residues), 151.4 bits, see alignment E=1.8e-48

Best Hits

KEGG orthology group: None (inferred from 99% identity to ppg:PputGB1_0743)

Predicted SEED Role

"Cytosine/purine/uracil/thiamine/allantoin permease family protein" in subsystem Purine Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PY9 at UniProt or InterPro

Protein Sequence (499 amino acids)

>PP_0709 Transporter, NCS1 nucleoside transporter family (Pseudomonas putida KT2440)
MSHSTGIETNGVEQIPADQRDASPLDLFRLIFGGANTFATAVLGSFPVLFGLSFQAGVWA
ILLGVGIGALILAPMGLFGALNGTNNAVSSGAHFGVHGRIVGSFLSLLTAVAFFSLSVWS
SGDALVGGAKRLAGLPETDLTLGLAYGLFAVLVLVVCIFGFRFMLWVNKIAVWASSLLFL
LGIVAFAGPFDAGYAGSVNLGQAGFWAAFVGAAILAMSNPVSFGAFLGDWSRYIPRETPK
ARIMLAVIAAQGATLIPFLFGLCTATLVATQAPDYIAANNYVGGLLAVAPSWFFLPVCLI
AVIGGMSTGTTALYGTGLDMSSVFPRLLSRAGATLLIGVLAIGFIFIGRFTFNLVQSVST
FAVLIITCTSPWMVIMILGLITRRGFYHADDLQVFTRGQRGGHYWFLHGWNWRGMGAWIP
SAAVGLCFVNLPGQFVGPLGDLAGGIDLSLPVTLGLAGVLYLLLLNLFPEPAGVYGPLGP
RWVRCKSTAHTPVTTAEMA