Protein Info for PP_0680 in Pseudomonas putida KT2440

Annotation: ATP-dependent protease-like protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 812 PF20437: LonC_helical" amino acids 92 to 122 (31 residues), 45.1 bits, see alignment (E = 1.6e-15) PF13654: AAA_32" amino acids 317 to 448 (132 residues), 175.3 bits, see alignment E=1.2e-55 PF20436: LonB_AAA-LID" amino acids 476 to 540 (65 residues), 67.6 bits, see alignment E=1.6e-22 PF05362: Lon_C" amino acids 650 to 753 (104 residues), 30.3 bits, see alignment E=6.1e-11

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppf:Pput_0711)

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type II" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.53

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88Q18 at UniProt or InterPro

Protein Sequence (812 amino acids)

>PP_0680 ATP-dependent protease-like protein (Pseudomonas putida KT2440)
MPDPVAARLRLAPEALTRRFSPEQFAFTHTDDLEPFRGVLGQERAVEALQFGVAMPRPGY
NVYVMGEPGTGRFSFVKRYLKAEGKRQQTPADWVYVNHFDDTREPRALELPSGSAAEFIS
DMGGLIDNLLSTFPAVFEHPSYQQKKGAIDRAFNQRYDRALDVIERASLEKDVALYRDAS
NVAFTPMADGKALDEAEFAQLPEEVREQFHEDIALLEERLNEELASLPQWKRESNNQLRQ
LNEETITLALQPLLAPLSEKYAENAAVCAYLQSVQLNLLRTLVEQLVDDSKSDAVARKLL
EEQYAPSLVVGHHADGGAPVVFEPHPTYDNLFGRIEYSTDQGALYTSYRQLRPGALHRAN
GGFLILEAEKMLGEPFVWDALKRALQSRKLKMESPIGELGRVATVSLQPQVIPLNVKLVI
IGSRQLYYALQDHDPDFQEMFRVLVDFDEDMPMVDENLEQFAQLLRTRTNEEGMAPLTSD
AVARLATYSARLAENQSRLSARIGDLFQLVSEADFIRQLASDEMTDAGHIERALKAKATR
TGRVSQRVLDDMLAGIILIDTEGAAIGKCNGLTVLEVGDSAFGMPARISATVYPGGSGIV
DIEREVNLGQPIHSKGVMILTGYLGSRYAQEFPLAISASIALEQSYGYVDGDSASLGEAC
TLISALSRTPLKQCFAITGSINQFGEVQAVGGVNEKIEGFFRLCEARGLTGEQGVIIPRA
NVATLMLDERVLQAVENGMFHVYAVSQADEALSLLVGEEAGVLDDKGLFTEGSVNARVVE
RLREIAEMISEEDIEKAEKERLEEVIAQAKPA