Protein Info for PP_0660 in Pseudomonas putida KT2440

Annotation: S-methyl-L-methionine transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 479 transmembrane" amino acids 25 to 45 (21 residues), see Phobius details amino acids 52 to 77 (26 residues), see Phobius details amino acids 99 to 127 (29 residues), see Phobius details amino acids 133 to 152 (20 residues), see Phobius details amino acids 164 to 186 (23 residues), see Phobius details amino acids 207 to 229 (23 residues), see Phobius details amino acids 250 to 268 (19 residues), see Phobius details amino acids 280 to 308 (29 residues), see Phobius details amino acids 340 to 361 (22 residues), see Phobius details amino acids 367 to 392 (26 residues), see Phobius details amino acids 413 to 433 (21 residues), see Phobius details amino acids 439 to 457 (19 residues), see Phobius details PF00324: AA_permease" amino acids 24 to 461 (438 residues), 418.5 bits, see alignment E=3.4e-129 PF13520: AA_permease_2" amino acids 26 to 439 (414 residues), 148.3 bits, see alignment E=3.5e-47

Best Hits

Swiss-Prot: 61% identical to YBGF_BACSU: Uncharacterized amino acid permease YbgF (ybgF) from Bacillus subtilis (strain 168)

KEGG orthology group: K03293, amino acid transporter, AAT family (inferred from 100% identity to ppf:Pput_0694)

MetaCyc: 58% identical to CP4-6 prophage; S-methyl-L-methionine transporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-486

Predicted SEED Role

"S-methylmethionine permease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88Q38 at UniProt or InterPro

Protein Sequence (479 amino acids)

>PP_0660 S-methyl-L-methionine transporter (Pseudomonas putida KT2440)
MSVQTTTTRNASAHTFKQDMQTRHIVMLALGGVIGTGLFLTSGYTVNQAGPLGAVIAYIL
GAIMVYLVMMCLGELAVHMPEVGSFSSYATRYLGPGTGYMVAWMYWLTWTVAIGSEFTAA
GILMVRWFPDTPVWMWSALFGAAVFISNIISVRSFAETEFWLSLVKVLAVIAFLVVGGGA
ILGGFEIQQAHSAGLGNFTREGLFPTGFWSIAMTLLAVAFAFSGTELIGIAAGETKDPEK
NVPKAIRTTVLRLALFFIGTIFVLATLLPREQAGVIESPFVMVFAMIGIPYAADIMNFVI
ITALLSAANSGLYAASRMLWTLSDQGNMPRRYATLSRRGTPFNAIVLSMAGGMASLLSSV
FAPDTIYLALVSISGLAVVVVWISIAASQIAFRRHYIASGGKLEDLKFRVRGYPFVPLGA
IFCCVLACVGIAFDPAQRVALYFGLPFIAWCYLAYWLTCKYRSRRTAPLEIVAPGSEAA