Protein Info for PP_0657 in Pseudomonas putida KT2440

Annotation: putative Amino acid ABC transporter, periplasmic amino acid-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 275 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF00497: SBP_bac_3" amino acids 43 to 270 (228 residues), 117.3 bits, see alignment E=2e-38

Best Hits

KEGG orthology group: K02030, polar amino acid transport system substrate-binding protein (inferred from 100% identity to ppu:PP_0657)

Predicted SEED Role

"Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1)" in subsystem Arginine and Ornithine Degradation (TC 3.A.1.3.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88Q41 at UniProt or InterPro

Protein Sequence (275 amino acids)

>PP_0657 putative Amino acid ABC transporter, periplasmic amino acid-binding protein (Pseudomonas putida KT2440)
MYMRFSSLLGAGLVLLATATQAFAGATLERVESRKEMVNVLMESYPPFSFLNDHNQLDGY
DVDVAKAVADKLGVKLRLETPSWDVIAAGRWSGRYDICICSMTPSKARAEVFDFPVEYYA
SPAVIVVNASDERIHDAKDLEGRKVGSISASSYESYLNKNLVIEGKEDQKIDYPFDFVQA
APYDTENVAFQDLGLGTGVRLDAIITNLVTAQPRLQQDKRFKLAGAPLYEEPNAVAIEKG
DPEWDAKVREVFAELKADGTLAKLSQKWIGADISK