Protein Info for PP_0652 in Pseudomonas putida KT2440

Annotation: Gluconate transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 transmembrane" amino acids 6 to 25 (20 residues), see Phobius details amino acids 32 to 54 (23 residues), see Phobius details amino acids 65 to 82 (18 residues), see Phobius details amino acids 104 to 133 (30 residues), see Phobius details amino acids 149 to 168 (20 residues), see Phobius details amino acids 188 to 210 (23 residues), see Phobius details amino acids 241 to 261 (21 residues), see Phobius details amino acids 279 to 299 (21 residues), see Phobius details amino acids 316 to 335 (20 residues), see Phobius details amino acids 357 to 385 (29 residues), see Phobius details amino acids 396 to 415 (20 residues), see Phobius details amino acids 436 to 458 (23 residues), see Phobius details TIGR00791: transporter, gluconate:H+ symporter (GntP) family" amino acids 8 to 456 (449 residues), 383.6 bits, see alignment E=6.9e-119 PF02447: GntP_permease" amino acids 9 to 455 (447 residues), 399.8 bits, see alignment E=1.5e-123 PF03600: CitMHS" amino acids 21 to 394 (374 residues), 39 bits, see alignment E=5.4e-14

Best Hits

Swiss-Prot: 39% identical to GNUT_PSEAE: Gluconate permease (gnuT) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_0652)

Predicted SEED Role

"Gluconate transporter family protein" in subsystem D-gluconate and ketogluconates metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88Q46 at UniProt or InterPro

Protein Sequence (461 amino acids)

>PP_0652 Gluconate transporter (Pseudomonas putida KT2440)
MTPLDIQLLLTALASVLVLVALIVSRLKMHPLLALLVVSIGVGFATHMAPGSIVSHLLTG
AGKTLGAVGVVIALGAMLGKILADAGVTEQVADVILKRTPDRMIPWAMMLVAFVIGIPMF
FEVGLVIMLPLIFSVARKLESKARFKGSAYVYVGVPVISALAAMHGMVPPHPGPLTAIAV
LKTSVGPTMLYGFLAAIPAMILGGPLYGMFISPRMNTRPDQALLDQFTLAEKADGQPRPG
VMVGMLAALLPAILMLVHAVAEMLLPKGNALLEMASFLGNPLIAMLLGVLFAGASLVLAR
GGDAEQLRDALGKSLKPIASIIMIIAGGGAFQEMLTSAKVGDAIVHLTQQSAFPPLILGW
LIAMLLSVSTGSATVGIVGAAGLLAPLAGADPSLNLPLLALSIGCGSLFFNYANHAGFWM
VKESFGMTMGEATKTISVVQSIVAVVGLMVVLMLNAAITIG