Protein Info for PP_0597 in Pseudomonas putida KT2440
Annotation: methylmalonate-semialdehyde dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 88% identical to BAUC_PSEAE: Putative 3-oxopropanoate dehydrogenase (bauC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 99% identity to ppg:PputGB1_0642)MetaCyc: 58% identical to malonate-semialdehyde dehydrogenase (acetylating) (Halomonas sp. HTNK1)
Malonate-semialdehyde dehydrogenase (acetylating). [EC: 1.2.1.18]
Predicted SEED Role
"Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.2.1.27)
MetaCyc Pathways
- β-alanine degradation II (2/2 steps found)
- L-valine degradation I (6/8 steps found)
- β-alanine degradation I (1/2 steps found)
- acrylate degradation I (3/5 steps found)
- propanoyl-CoA degradation II (3/5 steps found)
- 2,4-dinitrotoluene degradation (1/7 steps found)
- myo-inositol degradation I (1/7 steps found)
- myo-, chiro- and scyllo-inositol degradation (1/10 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Inositol phosphate metabolism
- Propanoate metabolism
- Valine, leucine and isoleucine degradation
- beta-Alanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.27
Use Curated BLAST to search for 1.2.1.18 or 1.2.1.27
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88Q97 at UniProt or InterPro
Protein Sequence (497 amino acids)
>PP_0597 methylmalonate-semialdehyde dehydrogenase (Pseudomonas putida KT2440) MSIVQHLIHGELVTKGERTADVFNPSTGQAVRKVELASRATVQEAIDSAKAAFPAWRNTP PAKRAQVMFRFKQLLEQNEAKISQMISEEHGKTLEDAAGELKRGIENVEFACAAPEVLKG EYSRNVGPNIDAWSDFQPLGVVAGITPFNFPAMVPLWMYPLAIACGNAFILKPSERDPSS TLYIAQLLLEAGLPKGILNVVHGDKEAVDALIEAPEVKALSFVGSTPIAEYIYAEGTKRG KRVQALGGAKNHAVLMPDADLDNAVSALMGAAYGSCGERCMAISVAVCVGDQVADALIAK LEPQIKALKIGAGTSCGLDMGPLVTAAARDKVVGYIDDGVAAGAKLVVDGRGFRVAGNED GYFVGGTLFDKVTPEMRIYKEEIFGPVLCVVRVNSLEQAMQLINDHEYGNGTCIFTRDGE AARLFCDEIEVGMVGVNVPLPVPVAYHSFGGWKRSLFGDLHAYGPDGVRFYTRRKAITQR WPQRASHEASQFAFPSL