Protein Info for PP_0589 in Pseudomonas putida KT2440

Annotation: Bcr/CflA family multidrug resistance transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 393 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details transmembrane" amino acids 43 to 60 (18 residues), see Phobius details amino acids 72 to 90 (19 residues), see Phobius details amino acids 96 to 117 (22 residues), see Phobius details amino acids 129 to 151 (23 residues), see Phobius details amino acids 159 to 179 (21 residues), see Phobius details amino acids 207 to 228 (22 residues), see Phobius details amino acids 245 to 266 (22 residues), see Phobius details amino acids 275 to 295 (21 residues), see Phobius details amino acids 301 to 324 (24 residues), see Phobius details amino acids 337 to 358 (22 residues), see Phobius details amino acids 364 to 383 (20 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 3 to 378 (376 residues), 302.5 bits, see alignment E=2.9e-94 PF07690: MFS_1" amino acids 15 to 349 (335 residues), 137.9 bits, see alignment E=4.1e-44 PF00083: Sugar_tr" amino acids 37 to 182 (146 residues), 46.3 bits, see alignment E=3.1e-16

Best Hits

KEGG orthology group: K07552, MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein (inferred from 100% identity to ppf:Pput_0628)

Predicted SEED Role

"Multidrug resistance transporter, Bcr/CflA family" in subsystem Copper homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QA5 at UniProt or InterPro

Protein Sequence (393 amino acids)

>PP_0589 Bcr/CflA family multidrug resistance transporter (Pseudomonas putida KT2440)
MNLRMVLILGALSAFGPLAIDFYLPAFPAMAQAFATDEKHVQATLAAYFLGLSIGQLAYG
PVADRFGRRKPLMFGVTLFTLASLACAYAPNLDTLVVARFIQALGGCAGMVLSRAIVSDK
CDPVASAKVFSQLMLVMGLAPILAPMLGGVLVNLAGWQSIFLALSLFSAGCLLAVSLGLP
ESLPEHIPRQPLSGALRQYLRLLADRVFLGHALTGGIAIAGMFAYIAGSPFVFIKLYGVP
AEHYGWLFGTNAAGFILVAQVNARLLAKRGPAFLLGRAVWLYLVAGLVLLGVAALRPAQL
WPLLVPLFICIASLGCIIPNASACAMSGQGARAGSASALMGCVQFSVAAGAAALVGLLHD
GSAVPMAIVISLCGALVVSVALLTRRLQAKAPA