Protein Info for PP_0586 in Pseudomonas putida KT2440

Annotation: cadmium translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 799 transmembrane" amino acids 157 to 175 (19 residues), see Phobius details amino acids 187 to 207 (21 residues), see Phobius details amino acids 219 to 239 (21 residues), see Phobius details amino acids 245 to 266 (22 residues), see Phobius details amino acids 404 to 423 (20 residues), see Phobius details amino acids 430 to 455 (26 residues), see Phobius details amino acids 744 to 762 (19 residues), see Phobius details amino acids 768 to 786 (19 residues), see Phobius details TIGR00003: copper ion binding protein" amino acids 8 to 68 (61 residues), 31.8 bits, see alignment 3.2e-11 amino acids 74 to 135 (62 residues), 43.2 bits, see alignment 8.8e-15 PF00403: HMA" amino acids 10 to 66 (57 residues), 51.6 bits, see alignment 1.5e-17 amino acids 75 to 133 (59 residues), 49 bits, see alignment 1e-16 TIGR01511: copper-translocating P-type ATPase" amino acids 201 to 787 (587 residues), 597.7 bits, see alignment E=6.4e-183 TIGR01525: heavy metal translocating P-type ATPase" amino acids 219 to 785 (567 residues), 625.1 bits, see alignment E=3.4e-191 TIGR01494: HAD ATPase, P-type, family IC" amino acids 257 to 767 (511 residues), 293.4 bits, see alignment E=6.4e-91 PF00122: E1-E2_ATPase" amino acids 285 to 464 (180 residues), 190.1 bits, see alignment E=3.9e-60 PF00702: Hydrolase" amino acids 483 to 698 (216 residues), 111.2 bits, see alignment E=1.3e-35

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 100% identity to ppu:PP_0586)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QA8 at UniProt or InterPro

Protein Sequence (799 amino acids)

>PP_0586 cadmium translocating P-type ATPase (Pseudomonas putida KT2440)
MPASTTYDLPISGMTCASCAGRVERALRKVTGAEQVSVNLTTEKARVLAPPASLPALVEA
VREAGYGVPTRTLELQIGGMTCASCVGRVERALGKLAGVEQVSVNLASERAHLEVLAALD
DNLLIDAVQKAGYSASLPQSTKDDQSAVQRRLRNERLAVGAALLLALPLVLPMLVQPFGL
HWMLPAWAQFLLATPVQFILGARFYIAAWKAVRAGAGNMDLLVALGTSAGYGLSLYQWAQ
APAGMAPHLYFEASAVVIALVLLGKYLESRAKRQTASAIRALEALRPERALRVVDGVEED
VAIAHLRVGDLVLVKPGERFPVDGVVEDGSSHADEALISGESLPVPKQPGDSVTGGAING
EGRLLVRTQALGTETVLARIIRLVEDAQAAKAPIQKLVDRVSQVFVPAVLVLALITLIGW
WLAGVPLETALINAVAVLVIACPCALGLATPAAIMAGTGVAARHGILIKDAEALERAHAV
NRVVFDKTGTLTSGSPQVVHSQALDGNSADLYRLAGALQRGSEHPLAKAVLVACAEQGLD
VPTVADSQSLTGRGIAGRVEGRELALGNRRLLDESGLQPGELAAQAQAWEAEGRTLSWLI
ERGKQPRVVGLFAFGDSLKPGAAQAIETLHAQHISSHLLTGDNRGSANVVAEALGIDDVH
AEVLPADKAATVAALKQEGVVAMVGDGINDAPALAAADIGIAMGGGTDVAMQAAGITLMR
GDPRLVPAALEISRKTYAKIRQNLFWAFIYNLIGIPLAALGYLNPVLAGAAMALSSVSVV
SNALWLKTWKPTSSTQEAP