Protein Info for PP_0574 in Pseudomonas putida KT2440

Annotation: DNA-binding response regulator, LuxR family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 258 PF00072: Response_reg" amino acids 10 to 123 (114 residues), 81 bits, see alignment E=1.1e-26 PF08281: Sigma70_r4_2" amino acids 182 to 229 (48 residues), 26.7 bits, see alignment 5.2e-10 PF00196: GerE" amino acids 189 to 242 (54 residues), 49.6 bits, see alignment E=3.6e-17

Best Hits

KEGG orthology group: None (inferred from 99% identity to ppf:Pput_0613)

Predicted SEED Role

"Serine phosphatase RsbU, regulator of sigma subunit" in subsystem SigmaB stress responce regulation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QC0 at UniProt or InterPro

Protein Sequence (258 amino acids)

>PP_0574 DNA-binding response regulator, LuxR family (Pseudomonas putida KT2440)
MSRSVSEVKVLVVDDQPVIVEQLSEFLETKGYACVTALSTDEAIEHYVADPTIGLLICDL
HMPDRDGIELVRALKEINGSQRTFEAIMLTGRAEKQDLIRAIREGFADYYQKPMDLDELL
AGVRRQEAALLERQRNLRDLGGLNQRLQELAESVDELYQDLEKARGQGTHRRASDAEESE
SELPAAFEKLSPRQLEVARLVGKGKTNYQIACELGITENTVKLYVSQVLRLTHMHNRTQL
ALALTPSSSPVHQRFTTH