Protein Info for PP_0539 in Pseudomonas putida KT2440

Annotation: glucose-regulated peptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 271 PF06167: Peptidase_M90" amino acids 5 to 249 (245 residues), 294.5 bits, see alignment E=3.4e-92

Best Hits

Swiss-Prot: 43% identical to MTFA_ERWT9: Protein MtfA (mtfA) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K09933, hypothetical protein (inferred from 100% identity to ppu:PP_0539)

Predicted SEED Role

"Inner membrane protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QF5 at UniProt or InterPro

Protein Sequence (271 amino acids)

>PP_0539 glucose-regulated peptidase (Pseudomonas putida KT2440)
MWSFSAWRRKRTLARYPITPQQWQAVRERLPMLDGISDAEDRWLREACILFLLDKHLTCL
PGVELDDEQRLMLAAQAQLPLLHLGELNWYQGFHEIILYPDDFKSPQRHRDASGVEHVWD
GEHSGEAWQQGPIILAWNGVQASGGWEAYNLVIHELAHKLDMLNGDANGLPPLHSGMPVD
EWASAMQQAYDDLNQQLDANPDAETAIDPYAAENPAEFFAVTSEYFFSAPDLLHQAYPKV
YHQLSLFYRQDPLARLCRLQAEHPEYRESHA