Protein Info for PP_0525 in Pseudomonas putida KT2440

Annotation: TonB-dependent receptor, B12 family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 641 PF07715: Plug" amino acids 71 to 175 (105 residues), 102.2 bits, see alignment E=3.3e-33 PF00593: TonB_dep_Rec_b-barrel" amino acids 203 to 605 (403 residues), 149.4 bits, see alignment E=4.5e-47

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 100% identity to ppu:PP_0525)

Predicted SEED Role

"Outer membrane vitamin B12 receptor BtuB" in subsystem Coenzyme B12 biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QG8 at UniProt or InterPro

Protein Sequence (641 amino acids)

>PP_0525 TonB-dependent receptor, B12 family (Pseudomonas putida KT2440)
MASRPGPFAGKPAPTGTAQCCGNPLELPMKKLACTTILCAPTFLLAAERDDALKLPDVLI
SASRQVESRTATSAANTVFTRTDIDRLQPSSVTDLLSRVPGVQVAPTGGRGSLPGIFIRG
TKAAQSLVLVDGVRIANATSGDSGLQFLDIDQIERIEVLRGSRSAVYGSDAIGGVIQIFT
RRSNGPGLQPRLRMAAGSNQTFQRSLGLSGGNDSTRFNLGASLDETAGIDSTGPSFASDG
DHDAYRNKALNLSLSHTFSERFEAGLNLLDSRGRREFDEPRGASPQVPYSNFAVSSIGSY
LDAQVTEQWSTRFELAHSENRDESRDKLSHSSAPFNTYRDQATWQNNLALGEHNSLLLGS
DWYEDRVHASEDFTEDSRWNHALFVQHRFHDEYFYTELGLRHDKNQQFGGQTTWSGSLTL
PLNEANDVLLSYSEGFRAPTFNDLYFPGYNNPELKPEHSKSYELQWRSQLSKNSKLEASL
YRTDLRDAIEYSERPENVGTARIDGFEIALSQEWNSWTSQLGLALIDPRNRENGHTLPRR
ARRTLNLDVDRQFDRFAVGATWQAVSSSYNDEGNSDPIAGYGIVGLRGSWVASRELTLDL
KLDNLLGKQYSRVHYNHQGNDYGYREERRTWLLSITWTPAL