Protein Info for PP_0489 in Pseudomonas putida KT2440

Annotation: formate dehydrogenase-O major subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1022 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details TIGR01553: formate dehydrogenase-N alpha subunit" amino acids 3 to 1021 (1019 residues), 1461.8 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 45 to 104 (60 residues), 59.7 bits, see alignment 3.3e-20 PF00384: Molybdopterin" amino acids 108 to 592 (485 residues), 108 bits, see alignment E=8.4e-35 PF01568: Molydop_binding" amino acids 899 to 1014 (116 residues), 47.1 bits, see alignment E=3.2e-16

Best Hits

Swiss-Prot: 66% identical to FDOG_ECOLI: Formate dehydrogenase-O major subunit (fdoG) from Escherichia coli (strain K12)

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 70% identity to bav:BAV3142)

MetaCyc: 63% identical to formate dehydrogenase N subunit alpha (Escherichia coli K-12 substr. MG1655)
FORMATEDEHYDROG-RXN [EC: 1.17.5.3]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.5.3 or 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A140FVZ1 at UniProt or InterPro

Protein Sequence (1022 amino acids)

>PP_0489 formate dehydrogenase-O major subunit (Pseudomonas putida KT2440)
MDLNRRQFFKVAAVGLGGSSLAALGMAPTPAFAEQVRHFKLAHTKETRNTCPYCSVGCGL
ILYSQGDAGKNVKQNIIHIEGDADHPVNRGTLCPKGAGLLDFIHSPSRLQYPEVRKPGSK
EWVRVSWDEALDRVADLMKQDRDANFIEKNAQGQTVNRWLTTGFLAASAASSEAGYLTHK
VIRATGMLGFDNQARVUHGPTVASLAPTYGRGAMTNHWSDIANANLVLVMGGNAAEAHPC
GFKWVTEAKAHNKARLIVVDPRFTRTASVADYYAPIRTGTDIAFMGGLINYLLSNDKIQH
EYVRNYTDVSFIVKENYGFEDGLFSGYDEAKRVYADKSGWGYELGEDGYAKVDPTLQHPR
CVFQLMKQHYSRYTPELASMTCGMPQDAMMKVWEEIASCSVPGKTMTILYALGWTQHSIG
AQIIRSAAMVQLLLGNVGMPGGGVNALRGHSNIQGLTDLGLLSNSLPGYLTLAGDAEQDY
AAYIDKRASKPLRPGQLSYWQNYGKFHVSLMKAWYGANATAENNWGYDWLPKLDVPAYDV
LRMFEMMGQGKVNGYMCQGFNPIAALPDKNRVTAALGKLKWLVIMDPLATETSEFWRNAG
PFNDVDTASIQTEVIRLPTTCFAEEDGSLVNSSRWLQWHWKGADGPGETRTDVQIMSELF
LRLRQRYQAEGGAYPDAIMNISWPYKIPEEPSPEELAKEMNGWAVADVTDPTGAVIKAGQ
QLAGFGQLKDDGSTASGCWIFAGSWTEQGNQMARRDNSDPYGMHQVQNWAWAWPANRRIL
YNRASSDPQGKPWDPEKKRLVWWNGKAWTGTDVPDFKVDSPPEAGMNPFIMNPEGVARFF
AIDKMAEGPFPEHYEPFETPIGINPLHPQNKKATSNPAGRIFDSVWDTLGTHGEFPYAAT
TYRLTEHFHFWSKHCRLNAIAQPEQFVEIGEVLANEKGIKAGDRVRVSSKRGHIDAVAVV
TKRIRPLQVNNQTVHQIGIPLHWGFTGATRHGYLTNTLVPFLGDGNTQTPESKSFLVKVE
KL