Protein Info for PP_0485 in Pseudomonas putida KT2440

Annotation: Single-stranded DNA-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 181 TIGR00621: single-stranded DNA-binding protein" amino acids 2 to 181 (180 residues), 193.1 bits, see alignment E=2.1e-61 PF00436: SSB" amino acids 5 to 107 (103 residues), 121.2 bits, see alignment E=9.4e-40

Best Hits

Swiss-Prot: 100% identical to SSB_PSEPK: Single-stranded DNA-binding protein (ssb) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03111, single-strand DNA-binding protein (inferred from 100% identity to ppg:PputGB1_0514)

MetaCyc: 56% identical to ssDNA-binding protein (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Single-stranded DNA-binding protein" in subsystem DNA repair, bacterial or DNA repair, bacterial RecFOR pathway or pVir Plasmid of Campylobacter

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QK5 at UniProt or InterPro

Protein Sequence (181 amino acids)

>PP_0485 Single-stranded DNA-binding protein (Pseudomonas putida KT2440)
MARGVNKVILVGTCGQDPEVRYLPNGNAVTNLSLATSEQWTDKQSGQKVERTEWHRVSLF
GKVAEIAGEYLRKGSQCYIEGKLQTREWEKDGIKRYTTEIIVDINGTMQLLGGRPQGQQQ
GGDPYNQGGGNYGGGQQQQYNQAPPRQQAQRPQQAPQRPAPQQPAPQPAADFDSFDDDIP
F