Protein Info for PP_0461 in Pseudomonas putida KT2440

Annotation: 50S ribosomal protein L16

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 137 TIGR01164: ribosomal protein uL16" amino acids 2 to 126 (125 residues), 196 bits, see alignment E=1.1e-62 PF00252: Ribosomal_L16" amino acids 4 to 132 (129 residues), 171.3 bits, see alignment E=5e-55

Best Hits

Swiss-Prot: 100% identical to RL16_PSEPW: 50S ribosomal protein L16 (rplP) from Pseudomonas putida (strain W619)

KEGG orthology group: K02878, large subunit ribosomal protein L16 (inferred from 94% identity to avn:Avin_06320)

MetaCyc: 79% identical to 50S ribosomal subunit protein L16 (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"LSU ribosomal protein L16p (L10e)" in subsystem Ribosome LSU bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QM8 at UniProt or InterPro

Protein Sequence (137 amino acids)

>PP_0461 50S ribosomal protein L16 (Pseudomonas putida KT2440)
MLQPKRTKFRKQMTGHNRGLALRGSKVSFGEFALKAVARGRLTARQIESARRALTRHVKR
GGKIWIRVFPDKPISKKPLEVRMGKGKGSVEYWVAQIQPGKVLYEIEGVSEELAREAFAL
AAAKLPLATSFVKRTVM