Protein Info for PP_0444 in Pseudomonas putida KT2440

Annotation: 50S ribosomal protein L1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 231 TIGR01169: ribosomal protein uL1" amino acids 3 to 228 (226 residues), 332 bits, see alignment E=8.1e-104 PF00687: Ribosomal_L1" amino acids 35 to 220 (186 residues), 215.1 bits, see alignment E=3.7e-68

Best Hits

Swiss-Prot: 100% identical to RL1_PSEP1: 50S ribosomal protein L1 (rplA) from Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)

KEGG orthology group: K02863, large subunit ribosomal protein L1 (inferred from 100% identity to ppf:Pput_0477)

MetaCyc: 71% identical to 50S ribosomal subunit protein L1 (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"LSU ribosomal protein L1p (L10Ae)" in subsystem Ribosome LSU bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QP4 at UniProt or InterPro

Protein Sequence (231 amino acids)

>PP_0444 50S ribosomal protein L1 (Pseudomonas putida KT2440)
MAKLTKRQKAIAEKIEAGKAYNFEEAATLLASLPAAKFVESYDIAVNLGVDPRKSDQVVR
SATVLPHGTGKTVRVAVFTQGPAAEAALAAGADRVGMDDLAAEMKGGDLNYDVVIASPDA
MRVVGQLGQVLGPRGLMPNPKVGTVTPDVATAVKNAKAGQVRYRTDKNGIIHTSVGKIGF
EADKLKENVEALIADLKRIKPASSKGIYVKRVTLSTTMGPGLVIDQSSLNV