Protein Info for PP_0433 in Pseudomonas putida KT2440

Annotation: Iron-sulfur cluster insertion protein ErpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 132 PF01521: Fe-S_biosyn" amino acids 27 to 127 (101 residues), 68.8 bits, see alignment E=2.3e-23 TIGR00049: iron-sulfur cluster assembly accessory protein" amino acids 28 to 131 (104 residues), 127.3 bits, see alignment E=1.3e-41

Best Hits

Swiss-Prot: 100% identical to ERPA_PSEPK: Iron-sulfur cluster insertion protein ErpA (erpA) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: None (inferred from 100% identity to ppg:PputGB1_0463)

Predicted SEED Role

"probable iron binding protein from the HesB_IscA_SufA family"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QQ5 at UniProt or InterPro

Protein Sequence (132 amino acids)

>PP_0433 Iron-sulfur cluster insertion protein ErpA (Pseudomonas putida KT2440)
MRVIEFYGGYRAGRINMSVETFTPTGLEFTHGAAQKVKNLVNEEGNERLKLRVFVTGGGC
SGFQYGFTFDEDVAEDDTIVEREGVSLVVDPMSFQYLAGAEVDYQEGLEGSRFVIKNPNA
ATTCGCGSSFSI