Protein Info for PP_0431 in Pseudomonas putida KT2440

Annotation: conserved membrane protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 141 transmembrane" amino acids 6 to 29 (24 residues), see Phobius details amino acids 50 to 71 (22 residues), see Phobius details amino acids 80 to 99 (20 residues), see Phobius details amino acids 120 to 138 (19 residues), see Phobius details PF03653: UPF0093" amino acids 2 to 141 (140 residues), 195.6 bits, see alignment E=5.2e-62 TIGR00701: TIGR00701 family protein" amino acids 3 to 141 (139 residues), 163.4 bits, see alignment E=1.7e-52 PF05425: CopD" amino acids 50 to 138 (89 residues), 36.5 bits, see alignment E=5.7e-13

Best Hits

Swiss-Prot: 48% identical to Y2845_RHOS4: UPF0093 membrane protein RHOS4_28450 (RHOS4_28450) from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

KEGG orthology group: K08973, putative membrane protein (inferred from 96% identity to ppw:PputW619_4772)

Predicted SEED Role

"Protoporphyrinogen IX oxidase, novel form, HemJ (EC 1.3.-.-)" (EC 1.3.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.-.-

Use Curated BLAST to search for 1.3.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QQ7 at UniProt or InterPro

Protein Sequence (141 amino acids)

>PP_0431 conserved membrane protein of unknown function (Pseudomonas putida KT2440)
MLYLWIKALHIVSVVCWFAGLFYLPRLFVYHAQSQDSISQERFVTMERKLYRGIMNPAMI
ATYVFGAWMLYLTPGWLSQGWLHAKLTLVILLTGYHHVCGAQRKRFANGTNTRSHVYYRW
FNEVPVLILLAIVILVVVKPF