Protein Info for PP_0416 in Pseudomonas putida KT2440

Annotation: Phosphoglycolate phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 272 PF00702: Hydrolase" amino acids 14 to 194 (181 residues), 116.2 bits, see alignment E=5e-37 PF13419: HAD_2" amino acids 16 to 199 (184 residues), 126.2 bits, see alignment E=3.3e-40 PF12710: HAD" amino acids 16 to 191 (176 residues), 61.5 bits, see alignment E=3.1e-20 TIGR01449: phosphoglycolate phosphatase, bacterial" amino acids 16 to 226 (211 residues), 230.7 bits, see alignment E=2.9e-72 TIGR01662: HAD hydrolase, family IIIA" amino acids 91 to 198 (108 residues), 45.7 bits, see alignment E=1.4e-15 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 95 to 199 (105 residues), 47.4 bits, see alignment E=4.8e-16 TIGR01549: HAD hydrolase, family IA, variant 1" amino acids 114 to 194 (81 residues), 33.2 bits, see alignment E=1.3e-11 PF13242: Hydrolase_like" amino acids 157 to 224 (68 residues), 43.3 bits, see alignment E=5.5e-15

Best Hits

Swiss-Prot: 100% identical to GPH_PSEPK: Phosphoglycolate phosphatase (PP_0416) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01091, phosphoglycolate phosphatase [EC: 3.1.3.18] (inferred from 100% identity to ppu:PP_0416)

Predicted SEED Role

"Phosphoglycolate phosphatase (EC 3.1.3.18)" in subsystem 2-phosphoglycolate salvage or Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 3.1.3.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.18

Use Curated BLAST to search for 3.1.3.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QS2 at UniProt or InterPro

Protein Sequence (272 amino acids)

>PP_0416 Phosphoglycolate phosphatase (Pseudomonas putida KT2440)
MSGFEQLFPGTLPRLVMFDLDGTLIDSVPDLAAAVDRMLLELGRPPAGLEAVRHWVGNGA
QVLVRRALAGGIDHADVDDALAEQALALFMDAYAESHELTVVYPGVRDTLRWLRKQGVEM
ALITNKPERFVGPLLDQMKIGNFFRWIIGGDTLPQKKPDPAALLFVMQMAGVTPQQSLFV
GDSRSDVQAAKAAGVQCVGLTYGYNHGRPIDAESPSLVIDDLRALLPGCADPATGITLAD
LQASQDRESTVAVTGKFWMKVIKALARWRWRA