Protein Info for PP_0409 in Pseudomonas putida KT2440

Annotation: Sensory box histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 795 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 275 to 296 (22 residues), see Phobius details PF00497: SBP_bac_3" amino acids 45 to 263 (219 residues), 51.4 bits, see alignment E=4.4e-17 PF00989: PAS" amino acids 331 to 432 (102 residues), 26.6 bits, see alignment E=2.4e-09 PF13188: PAS_8" amino acids 331 to 381 (51 residues), 27.1 bits, see alignment 1.3e-09 TIGR00229: PAS domain S-box protein" amino acids 331 to 445 (115 residues), 43.5 bits, see alignment E=1.6e-15 amino acids 477 to 578 (102 residues), 34.2 bits, see alignment E=1.2e-12 PF08448: PAS_4" amino acids 333 to 441 (109 residues), 31.6 bits, see alignment E=7.4e-11 PF13426: PAS_9" amino acids 338 to 438 (101 residues), 39.1 bits, see alignment E=3.6e-13 PF08447: PAS_3" amino acids 480 to 564 (85 residues), 55.8 bits, see alignment E=2.1e-18 PF07730: HisKA_3" amino acids 599 to 665 (67 residues), 52.3 bits, see alignment E=3.2e-17 PF02518: HATPase_c" amino acids 705 to 794 (90 residues), 52 bits, see alignment E=4.2e-17

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_0409)

Predicted SEED Role

"Probable two-component sensor, near polyamine transporter" in subsystem Polyamine Metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QS9 at UniProt or InterPro

Protein Sequence (795 amino acids)

>PP_0409 Sensory box histidine kinase (Pseudomonas putida KT2440)
MKRVRRLLVIGCLWLPLIAWAKPEALPSVVLEPDQQQWLDTHRSLRVGLVLQAPYAQFDR
RLQQLYGANVELVNSLAQTLRLDLTWRNFTDQGSLEHALQSGEIDFAPGLTQTPASLRLW
LFSDPYMRVPQLVVGPRTGAMAVELEKLEAEQRVAVRMPSPLADYLRGNYGNLNLQGVPD
DREALQLVVGGQASFAVLDEAQLSRLSRESEFGELAVVGDIGLPQLLRIGSRRDWPMLAD
VLERGLQAVPAKELEQLHQRWLQPKYPRLSESPGFWQNMALLFGLLLLCALATLVWQRRQ
QRQLERSLLAARESLVERQVREEALRLSQFAIDQSTVGILWVNWDSHVRYANHAAERMLG
YSEGALLERPLSDFEPSLNMDRWLELWKGARTGAGGVGQFETQCRRADQSLLPVELSLSF
LRFRDSEYLVVYLADVTERHRALAALRESEARLKGIAGNVPGLVFRLERDPAEGDLEFPY
ISEGSEALVGYAPSEIQHPQMGLRNLVHPEDRADYHRVQDLALASDQDWSWQGRILTRQG
EQRWADIKASARHLGNGQVVWDGVVWDITQGKRAELALAKSQEQLRELSAHLESVREEEK
ARIAREVHDELGQMLTVLKLEVSMCELAYAELDPGLNDRLASMKRLIAQLFQLVRDVATA
LRPPILDAGIASAIEWQARRFEARTQIPCLVQVPDNLPALSDAKATGLFRILQEALTNVM
RHAQAHSVEIELVREGGQLRMTVSDDGQGFCREQTRPTSFGLVGVRERVLMLGGSMTLES
EPGEGTSLSVAIPLE