Protein Info for PP_0403 in Pseudomonas putida KT2440

Annotation: peptidyl-prolyl cis-trans isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 439 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF09312: SurA_N" amino acids 30 to 147 (118 residues), 144 bits, see alignment E=8.4e-46 PF13624: SurA_N_3" amino acids 31 to 146 (116 residues), 52.1 bits, see alignment E=2.4e-17 PF13623: SurA_N_2" amino acids 32 to 110 (79 residues), 33.2 bits, see alignment E=1.6e-11 PF13145: Rotamase_2" amino acids 157 to 281 (125 residues), 41.3 bits, see alignment E=9.1e-14 amino acids 289 to 397 (109 residues), 34.1 bits, see alignment E=1.5e-11 PF13616: Rotamase_3" amino acids 171 to 278 (108 residues), 56.2 bits, see alignment E=1.8e-18 amino acids 287 to 387 (101 residues), 98.9 bits, see alignment E=9.9e-32 PF00639: Rotamase" amino acids 184 to 276 (93 residues), 73.4 bits, see alignment E=8.7e-24 amino acids 294 to 384 (91 residues), 91.1 bits, see alignment E=2.7e-29

Best Hits

Swiss-Prot: 100% identical to SURA_PSEPK: Chaperone SurA (surA) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03771, peptidyl-prolyl cis-trans isomerase SurA [EC: 5.2.1.8] (inferred from 100% identity to ppf:Pput_0437)

MetaCyc: 42% identical to chaperone SurA (Escherichia coli K-12 substr. MG1655)
Peptidylprolyl isomerase. [EC: 5.2.1.8]

Predicted SEED Role

"Survival protein SurA precursor (Peptidyl-prolyl cis-trans isomerase SurA) (EC 5.2.1.8)" in subsystem Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8)

Isozymes

Compare fitness of predicted isozymes for: 5.2.1.8

Use Curated BLAST to search for 5.2.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QT4 at UniProt or InterPro

Protein Sequence (439 amino acids)

>PP_0403 peptidyl-prolyl cis-trans isomerase (Pseudomonas putida KT2440)
MKTKLIDRLRPVLLGVALLSGAVHAAVQPLDRVVAIVDNDVVMQSQLDQRVHEVQQTIAK
RGGGVPPTSALEQQVLERLIVENLQLQIGERSGIRITDEELNQAIGTIAQRNGMSLDQFR
AALARDGLSFDDAREQVKREMIISRVRQRRVAERIQVSEQEVKNFLASDLGKMQMSEEYR
LANILIPTPEAANSDDIQKAARKVGDVYQQLRQGADFGQMAIANSASENALEGGEMGWRK
AGQLPPDFAKMLSSMPVGEITQPIRIPNGFIILKLEEKRGGSENVLRDEVHVRHILIKPS
EIRSEAATEQLAERLYDRIKNGEDFGELAKSFSEDPGSALNGGDLNWVDPNSLVPEFREQ
MANAQQGVVTKPFKTQYGWHVLEVLGRRATDSTEQAREQQALSVLRNRKYDEELQTWLRQ
IRDEAYVEIKLPGADQAAQ