Protein Info for PP_0399 in Pseudomonas putida KT2440

Annotation: Bis(5'-nucleosyl)-tetraphosphatase, symmetrical

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to APAH_PSEP1: Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (apaH) from Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)

KEGG orthology group: K01525, bis(5'-nucleosyl)-tetraphosphatase (symmetrical) [EC: 3.6.1.41] (inferred from 100% identity to ppf:Pput_0433)

MetaCyc: 50% identical to diadenosine tetraphosphatase (Escherichia coli K-12 substr. MG1655)
Bis(5'-nucleosyl)-tetraphosphatase (symmetrical). [EC: 3.6.1.41]; Guanosine-5'-triphosphate,3'-diphosphate diphosphatase. [EC: 3.6.1.41, 3.6.1.40]

Predicted SEED Role

"Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41)" (EC 3.6.1.41)

MetaCyc Pathways

ppGpp metabolism (6/6 steps found)

KEGG Metabolic Maps

Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.40

Use Curated BLAST to search for 3.6.1.40 or 3.6.1.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QT8 at UniProt or InterPro

Protein Sequence (288 amino acids)

>PP_0399 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (Pseudomonas putida KT2440)
MATYAVGDLQGCLQPLKCLLDRVSFNPAVDRLWLVGDLVNRGPESLETLRFLYSIRHSLV
CVLGNHDLHLLAAWHNVERLKKSDTLREIIEAPDADQLFDWLRQQKLLHYDEPRGIALVH
AGIPPQWTLGKALELAAEVEEVLRDDTRLQLYLDGMYGNEPNKWSKNLAGVERLRVITNY
FTRMRFCTADGKLDLKSKEGLGSAPKGYKAWYAHKDRRSRHVKIIFGHWAALQGEVTEPD
VIALDTGCVWGGAMTLYNVDSGEYHRCDCADDGTLRQPAQPTTLNDHT