Protein Info for PP_0383 in Pseudomonas putida KT2440

Annotation: Lysine 2-monooxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 560 PF13450: NAD_binding_8" amino acids 50 to 117 (68 residues), 39.5 bits, see alignment E=8.7e-14 PF01593: Amino_oxidase" amino acids 55 to 530 (476 residues), 157.9 bits, see alignment E=7.9e-50

Best Hits

KEGG orthology group: K00466, tryptophan 2-monooxygenase [EC: 1.13.12.3] (inferred from 100% identity to ppf:Pput_0407)

MetaCyc: 100% identical to L-lysine monooxygenase monomer (Pseudomonas putida)
Lysine 2-monooxygenase. [EC: 1.13.12.2]

Predicted SEED Role

"Lysine 2-monooxygenase (EC 1.13.12.2)" in subsystem Lysine degradation (EC 1.13.12.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.13.12.2 or 1.13.12.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QV1 at UniProt or InterPro

Protein Sequence (560 amino acids)

>PP_0383 Lysine 2-monooxygenase (Pseudomonas putida KT2440)
MNKKNRHPADGKKPITIFGPDFPFAFDDWLEHPAGLGSIPAERHGEEVAIVGAGIAGLVA
AYELMKLGLKPVVYEASKLGGRLRSQAFNGTDGIVAELGGMRFPVSSTAFYHYVDKLGLE
TKPFPNPLTPASGSTVIDLEGQTYYAEKPTDLPQLFHEVADAWADALESGAQFADIQQAI
RDRDVPRLKELWNKLVPLWDDRTFYDFVATSRSFAKLSFQHREVFGQVGFGTGGWDSDFP
NSMLEIFRVVMTNCDDHQHLVVGGVEQVPQGIWRDVPERCVHWPEGTSLSTLHGGAPRTG
VKRIARAADGRLAVTDNWGDTRHYSAVLATCQTWLLTTQIDCEESLFSQKMWMALDRTRY
MQSSKTFVMVDRPFWKDKDPETGRDLLSMTLTDRLTRGTYLFDNGNDKPGVICLSYSWMS
DALKMLPHPVEKRVQLALDALKKIYPKTDIAGHIIGDPITVSWEADPYFLGAFKGALPGH
YRYNQRMYAHFMQQDMPAEQRGIFIAGDDVSWTPAWVEGAVQTSLNAVWGIMNHFGGHTH
PDNPGPGDVFNEIGPIALAD