Protein Info for PP_0365 in Pseudomonas putida KT2440

Annotation: Malonyl-[acyl-carrier protein] O-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 272 PF01209: Ubie_methyltran" amino acids 19 to 152 (134 residues), 41.1 bits, see alignment E=4.8e-14 TIGR02072: malonyl-acyl carrier protein O-methyltransferase BioC" amino acids 22 to 269 (248 residues), 250.6 bits, see alignment E=9.7e-79 PF13489: Methyltransf_23" amino acids 39 to 198 (160 residues), 41 bits, see alignment E=5.9e-14 PF13847: Methyltransf_31" amino acids 57 to 170 (114 residues), 55 bits, see alignment E=2.9e-18 PF01728: FtsJ" amino acids 57 to 136 (80 residues), 24.2 bits, see alignment E=1e-08 PF08242: Methyltransf_12" amino acids 58 to 150 (93 residues), 48.2 bits, see alignment E=5.5e-16 PF08241: Methyltransf_11" amino acids 58 to 152 (95 residues), 80.9 bits, see alignment E=3.1e-26 PF13649: Methyltransf_25" amino acids 58 to 148 (91 residues), 66.1 bits, see alignment E=1.3e-21

Best Hits

Swiss-Prot: 69% identical to BIOC_PSEA7: Malonyl-[acyl-carrier protein] O-methyltransferase (bioC) from Pseudomonas aeruginosa (strain PA7)

KEGG orthology group: K02169, biotin synthesis protein BioC (inferred from 100% identity to ppu:PP_0365)

Predicted SEED Role

"Biotin synthesis protein BioC"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88QW9 at UniProt or InterPro

Protein Sequence (272 amino acids)

>PP_0365 Malonyl-[acyl-carrier protein] O-methyltransferase (Pseudomonas putida KT2440)
MTDLSRPTLPGALPDKRQVAASFSRAAASYDSVAALQRAVGLSLLEQLPAGLQPSHWLDL
GSGTGHFSRMLAERFAQAGGVAVDIAEGMLLHARHVKGGAQYHVVGDAERLPLRDASVDL
VFSSLAVQWCDQFASVLAEAQRVLRPGGVLAFSSLCVGTLDELRASWQAVDGLVHVNRFR
RFEDYQRLCAASGFEQLELERCPHVLHYPDVRSLTHELKALGAHNLNPGRPSGLTGRARM
QGLLQAYEAFRQPAGLPATYQVVYGVLRKPLA