Protein Info for PP_0313 in Pseudomonas putida KT2440

Annotation: putative electron transfer flavoprotein beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 256 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF01012: ETF" amino acids 26 to 181 (156 residues), 54.9 bits, see alignment E=5.1e-19

Best Hits

KEGG orthology group: K03521, electron transfer flavoprotein beta subunit (inferred from 98% identity to ppw:PputW619_4894)

Predicted SEED Role

"Electron transfer flavoprotein, beta subunit" in subsystem Acetyl-CoA fermentation to Butyrate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88R21 at UniProt or InterPro

Protein Sequence (256 amino acids)

>PP_0313 putative electron transfer flavoprotein beta subunit (Pseudomonas putida KT2440)
MSTKVISLVSIGAHPSSGRARRAEQDARAVELGLQLAGDNLQVVHAGNPQEEALRAYLGM
GLDHLDVLEQPAGADVLGVLGDYLRDAGAQLVLTGSQAETGEGSGMLPFLLAEKLGWPLI
VGLAEVESIDNGTAQVLQALPRGQRRRLKVRLPLLATVDNAAPKPRQSAFGPARRGVLAA
RNVAIVEDELLAEAELQPARPRPKRLKVIKAKSGADRMKAATAKASGGGGKVLKDVSPQE
GAEAILKLLVEEGVLR