Protein Info for PP_0312 in Pseudomonas putida KT2440

Annotation: electron transfer flavoprotein, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 410 PF01012: ETF" amino acids 102 to 213 (112 residues), 49 bits, see alignment E=6.4e-17 PF00766: ETF_alpha" amino acids 283 to 358 (76 residues), 86.3 bits, see alignment E=1.1e-28

Best Hits

KEGG orthology group: K03522, electron transfer flavoprotein alpha subunit (inferred from 98% identity to ppf:Pput_0333)

Predicted SEED Role

"Electron transfer flavoprotein, alpha subunit" in subsystem Acetyl-CoA fermentation to Butyrate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88R22 at UniProt or InterPro

Protein Sequence (410 amino acids)

>PP_0312 electron transfer flavoprotein, alpha subunit (Pseudomonas putida KT2440)
MSDIIRRDPRAEWIARNRLHPLHAAMQTQQTSWMGPNGIIRKNPHAIAAGFVGPAGIKRI
DRSGAQQGTGAGGRRTAAAEVKLPLHQVATPAFYIAVVPDMVGGRLSSHDRDLLGLAHSL
AGSDGAVLAVVFNEHKESNFSTAGVDRLLVIEGEAFEGYAPEQLVQGLRAVDNQFAPRHW
LLPDSRTGGGELGRRLGAALGERPATRVWQVKDGQCIGRAGAGQQDLQRTVPRLILAAAE
CAEPVSETLHEALPVELSTSVVRSLPRIEDLGSVAVDPATIAMAEAEFIVSGGNGVKDWD
LYHKATAALGATEGASRVAVDDGFMPRNRQVGATGTWVTARVYVAVGISGAIQHLQGIGA
CDKVVAINMDPGCDMIKRADLSVIGDSSAILKALIEAVDNYRSGSQRDAA