Protein Info for PP_0287 in Pseudomonas putida KT2440

Annotation: conserved exported protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 748 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF05170: AsmA" amino acids 1 to 630 (630 residues), 389.5 bits, see alignment E=3.6e-120 PF13502: AsmA_2" amino acids 478 to 661 (184 residues), 68.9 bits, see alignment E=5e-23

Best Hits

KEGG orthology group: K07289, AsmA protein (inferred from 100% identity to ppu:PP_0287)

Predicted SEED Role

"A/G-specific adenine glycosylase (EC 3.2.2.-)" in subsystem DNA repair, bacterial (EC 3.2.2.-)

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.-

Use Curated BLAST to search for 3.2.2.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88R47 at UniProt or InterPro

Protein Sequence (748 amino acids)

>PP_0287 conserved exported protein of unknown function (Pseudomonas putida KT2440)
MKAFGKILGLGLLGLLLIIVALGFALTHLFDPNDYKDEIRQLARDKAHVELTLNGDIGWS
LFPWLGLELHEASIATLNKPKEPFADLQMLGLSVRVLPLLRREVQMSDVRVEGLNLTLAR
DEQGHGNWEDIGKPLPAQNGASTDASAQAPAEQTPATTNSNDRAIKLDIDSLTVNNARVQ
YTDAKTGHSYSAESIQLSTGPIHEGANIALKASAFISASQPSIKARTELAGELRFDRKLK
RYNFEDMRLSGETSGEPTGGKTVTFAAQGQLLVDLAANVASWNGLKVSANQLRALGELNL
RDLDKAPQLSGGLSIAQFNLRAFLESIGHPLPATNDPAAFAKLELVTRLLGTPSSLALED
LAVKLDDSTFSGRVAVEDFAKQALRLQLKGDTFDADRYLPAKSEEAKGATAARQAEVKQQ
EASAVAGAGTTPLPNAPTQVAWSDDKLLPVDRLRALDLQADLAFGSLTLDKLPIESAQLK
AVGQGGLLTLQTLRGELYNGTFEAKGTVDVRPAVPQLGVNTNIQRVPVEHFIKTEGKEQT
PPVKGLLTLTSDLTATGNSQKALVDTLNGSANFAINDGVLVNANLEQQLCQAIATLNRKT
LSSEPRGKDTPFQELRGSLVVRNGVASNPDLKARIPGLTVNGHGDLDLRVLGMDYNIGIV
VEGDQRAMPDPACQVNERYVGVEVPLRCRGPLELGAKACRLDQNGLGKVAAKLAGNRLKD
KIDEKLDEKLGDKVSPELKDALKGLFNR