Protein Info for PP_0284 in Pseudomonas putida KT2440

Annotation: gamma-aminobutyrate permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 transmembrane" amino acids 20 to 40 (21 residues), see Phobius details amino acids 46 to 64 (19 residues), see Phobius details amino acids 85 to 107 (23 residues), see Phobius details amino acids 116 to 120 (5 residues), see Phobius details amino acids 125 to 143 (19 residues), see Phobius details amino acids 154 to 177 (24 residues), see Phobius details amino acids 198 to 220 (23 residues), see Phobius details amino acids 240 to 261 (22 residues), see Phobius details amino acids 286 to 307 (22 residues), see Phobius details amino acids 335 to 354 (20 residues), see Phobius details amino acids 360 to 379 (20 residues), see Phobius details amino acids 400 to 422 (23 residues), see Phobius details amino acids 428 to 447 (20 residues), see Phobius details TIGR01773: GABA permease" amino acids 6 to 450 (445 residues), 743.7 bits, see alignment E=3.5e-228 PF13520: AA_permease_2" amino acids 16 to 421 (406 residues), 138.2 bits, see alignment E=5.7e-44 PF03845: Spore_permease" amino acids 16 to 257 (242 residues), 28.1 bits, see alignment E=1.4e-10 PF00324: AA_permease" amino acids 17 to 430 (414 residues), 433.1 bits, see alignment E=2e-133

Best Hits

Swiss-Prot: 71% identical to BAUD_PSEAE: Probable GABA permease (bauD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03293, amino acid transporter, AAT family (inferred from 99% identity to ppg:PputGB1_0307)

MetaCyc: 68% identical to 4-aminobutanoate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-384; TRANS-RXN-57

Predicted SEED Role

"transport permease protein of gamma-aminobutyrate"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88R50 at UniProt or InterPro

Protein Sequence (461 amino acids)

>PP_0284 gamma-aminobutyrate permease (Pseudomonas putida KT2440)
MSGNNSNDLAQGLKQRHVTMLSIAGVIGAGLFVGSGHAIAAAGPAVLLAYAAAGTLVVLV
MRMLGEMAVASPDTGSFSTYADRAIGRWAGFTIGWLYWWFWVLVIPLEANAAAAILHAWF
PAVDLWAFSLIITLALTLTNLCSVKNYGEFEFWFALLKVLAIIGFIAVGCAALFGFVPSS
QVSGTSHLFDTQGFMPNGLGAVLAAMLTTMFSFMGTEIVTIAAAESKDPGKQISRATNSV
IWRICLFYLVSIFLVVALVPWNDPALAETGSYQTVLSRIGVPNAKLIVDIVVLIAVTSCL
NSALYTSSRMLFSLSKRGDAPAIAQRTTKAATPHVAVLLSTAAAFLCVFANFVAPAQVFE
FLLASSGAIALLVYLVIAVSQLRMRAQREARGEKITFKMWLFPGLTWATIAFIVAILVVM
ALREDHRAEIIATALLSIGVVAAGLLVHRKREAAGRAALDS