Protein Info for PP_0269 in Pseudomonas putida KT2440
Annotation: putative Glutamate synthase, large subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 100% identity to ppu:PP_0269)Predicted SEED Role
"Ferredoxin-dependent glutamate synthase (EC 1.4.7.1)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.7.1)
MetaCyc Pathways
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- L-glutamate and L-glutamine biosynthesis (6/7 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- superpathway of ammonia assimilation (plants) (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- ammonia assimilation cycle II (2/2 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-glutamate biosynthesis V (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- nitrogen remobilization from senescing leaves (6/8 steps found)
- GABA shunt II (3/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.4.7.1
Use Curated BLAST to search for 1.4.7.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88R65 at UniProt or InterPro
Protein Sequence (555 amino acids)
>PP_0269 putative Glutamate synthase, large subunit (Pseudomonas putida KT2440) MKHSLPSRYACLVLCLVFTFASLPLLAQHAWLWPVTLITALLSLVGLNDLRQSHHAVRRN YPILGNIRYLIETIRPEIRQYLIEGDDDKLPFSRSQRSLVYARAKNESAEKAFGTLNDAY KPGFEFISHSMLPVATPDPASFRIAIGGPQCRMPYSASIFNISAMSFGALSANAIAALNR GARMGRFAHDTGEGSISPYHREHGGDLIWEIGSGYFGCRTEDGRFDPQRFAEQARSAQVK MIEIKLSQGAKPGHGGILPGHKVSPEIAETRGVRAGEDCISPAAHSAFRTPVELLQFVAG LRELSGGKPVGFKFCLGHPWEFMGIAKAMLATGITPDFIVVDGKEGGTGAAPREFSDNMG VPMREGLMFVHNTLVGLNLRSSIRIGAAGKIVSAFDIASVLAIGADWVNSARGFMFAIGC IQSQSCHTNKCPTGVATQDPLRQRALVVPDKAERVASFHRNTLHALAEMLAAAGLEHPSE LKPKHLARRVSPSEIGLFSDLHTFLKPGELLSGSIESEFYARMWRMARSDSFAPETVSPA PAQTVTVRRKETAPA