Protein Info for PP_0251 in Pseudomonas putida KT2440

Annotation: conserved membrane protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 264 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details amino acids 62 to 87 (26 residues), see Phobius details amino acids 99 to 117 (19 residues), see Phobius details amino acids 166 to 206 (41 residues), see Phobius details amino acids 213 to 232 (20 residues), see Phobius details amino acids 241 to 262 (22 residues), see Phobius details PF01569: PAP2" amino acids 150 to 230 (81 residues), 35.5 bits, see alignment E=3.8e-13

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_0251)

Predicted SEED Role

"Membrane-associated phospholipid phosphatase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88R82 at UniProt or InterPro

Protein Sequence (264 amino acids)

>PP_0251 conserved membrane protein of unknown function (Pseudomonas putida KT2440)
MDNRPLFQARWSWGPLAASTLLPIALLCFWLWPFGQILCLTFDEWLFRSLNAPLADSTTW
RYIWTIGSLRPFDIVVGLILLAVLIRGDWVFKAAQVRQAFFGFLVTLLLLVVIRALFSKW
VDAAGWQHKSPSMIFDDVVHLSDYYPNLEAAWELKDRSSKSFPGDHASVLLIWALFMSVF
SRGLAQYLMVWGLAALFMLPRLVAGAHWGQDDYIGGLLMAVLALGWSYYTPLAAKGSEAL
MRWTAPLFKLLSTLPLVGRMAVLR