Protein Info for PP_0245 in Pseudomonas putida KT2440

Annotation: putative transcriptional accessory protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 861 signal peptide" amino acids 22 to 23 (2 residues), see Phobius details transmembrane" amino acids 24 to 31 (8 residues), see Phobius details PF09371: Tex_N" amino acids 90 to 163 (74 residues), 110.2 bits, see alignment E=1.1e-35 PF22706: Tex_central_region" amino acids 218 to 394 (177 residues), 248.3 bits, see alignment E=1.7e-77 PF16921: Tex_YqgF" amino acids 411 to 536 (126 residues), 183.3 bits, see alignment E=8e-58 PF12836: HHH_3" amino acids 576 to 640 (65 residues), 99.8 bits, see alignment 2.6e-32 PF17674: HHH_9" amino acids 646 to 715 (70 residues), 91 bits, see alignment E=2.5e-29 PF23459: S1_RRP5" amino acids 733 to 803 (71 residues), 31 bits, see alignment E=1e-10 PF00575: S1" amino acids 733 to 805 (73 residues), 71.6 bits, see alignment E=2e-23

Best Hits

Swiss-Prot: 63% identical to TEX_BORPE: Protein tex (tex) from Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)

KEGG orthology group: K06959, uncharacterized protein (inferred from 100% identity to ppu:PP_0245)

Predicted SEED Role

"Transcription accessory protein (S1 RNA-binding domain)" in subsystem ZZ gjo need homes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88R88 at UniProt or InterPro

Protein Sequence (861 amino acids)

>PP_0245 putative transcriptional accessory protein (Pseudomonas putida KT2440)
MRILSPSAVFGVPVMNNSSDIAAHYGVAIAARCVSIVSRFFPRRQFRLAGRHHRKPFAMG
IMRGFHPAPPGKKPTGDPRIHKCQVVYMDSINSRIAEELGVRPQQVEAAVSLLDEGSTVP
FIARYRKEVTGSLDDTQLRHLEERLRYLRELDERRASILSSIEEQGKLTPELAREIKLAD
TKTRLEDLYLPYKQKRRTKGQIALEAGLGELADGLFNDPTLAPESEAARFVNAEKGVADV
KAALEGAKYILMERFAEDAALLDKLRSYLKQEAVLSARVVAGKEEEGAKFRDYFAHDELL
RTAPSHRALAIFRGRNEGVLSVSLKVGEELPGTLHPCELMIGNHVGIENRSRPADKWLGE
VVRWTWKVKLYTHLETDLFGELRDSAEGEAINVFAHNLHDLLLAAPAGPRATLGFDPGLR
TGCKIAVVDATGKLLDHTTVYPHAPKNDWDRTISIMAALCAKHSVELIAIGNGTASRESD
KLVTELVKKYPALKITKIMVSEAGASVYSASELAAREFPDLDVSIRGAVSIARRLQDPLA
ELVKIDPKSIGVGQYQHDVSQLKLARGLDAVVEDCVNAVGVDVNTASVALLTRISGLNAT
LAQNIVAHRDANGPFATRAALKKVSRLGEKTFEQAAGFLRVMNGDNPLDASAVHPEAYPL
VQRIAADTDRDIRSLIGDSSFLKRLDPKKFTDETFGLPTVTDILQELDKPGRDPRPEFKT
ATFQDGVEDLKDLEPGMILEGVVTNVTNFGAFVDIGVHQDGLVHISALSEKFVKDPREAV
KAGDVVKVKVMEVDIPRKRVGLSMRMSDTPGEKVEGNRGGNRGNGGNRQQQAPRQRETAT
AAPANNAMAALFANAKQLKKK