Protein Info for PP_0227 in Pseudomonas putida KT2440

Annotation: periplasmic cystine-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 264 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF00497: SBP_bac_3" amino acids 40 to 259 (220 residues), 156.8 bits, see alignment E=2.7e-50 PF10613: Lig_chan-Glu_bd" amino acids 60 to 133 (74 residues), 38.8 bits, see alignment E=9.4e-14

Best Hits

Swiss-Prot: 62% identical to FLIY_ECOLI: L-cystine-binding protein FliY (fliY) from Escherichia coli (strain K12)

KEGG orthology group: K02424, cystine transport system substrate-binding protein (inferred from 100% identity to ppf:Pput_0242)

MetaCyc: 62% identical to cystine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-290 [EC: 7.4.2.12]; 7.4.2.12 [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]

Predicted SEED Role

"Cystine ABC transporter, periplasmic cystine-binding protein FliY"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RA6 at UniProt or InterPro

Protein Sequence (264 amino acids)

>PP_0227 periplasmic cystine-binding protein (Pseudomonas putida KT2440)
MSKFAKPLLNASLALLLGAGLLSQAFAGEQLKTIQEKGVINVGLEGTYPPFSFQDENGKL
TGFEVELSELLAKELGVKAKIQPTKWDGILAALESKRLDVVVNQVTISEERKKKYDFSEP
YTVSGIQALILKKKAEQLNIKGAQDLAGKKVGVGLGTNYEQWVKKDVPQADVRTYEDDPS
KFADLRNGRIDAILIDRLAALEYAQKAKDTELAGDAFSRLESGVALRKGEPELLAAINKA
IDKLKADGTLAKLSEKYFGADVTK