Protein Info for PP_0166 in Pseudomonas putida KT2440

Annotation: HlyD family secretion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 458 transmembrane" amino acids 43 to 64 (22 residues), see Phobius details TIGR01843: type I secretion membrane fusion protein, HlyD family" amino acids 42 to 458 (417 residues), 436.4 bits, see alignment E=6e-135 PF00529: CusB_dom_1" amino acids 57 to 404 (348 residues), 27.8 bits, see alignment E=4e-10 PF13533: Biotin_lipoyl_2" amino acids 83 to 120 (38 residues), 26.6 bits, see alignment 8.3e-10 PF13437: HlyD_3" amino acids 309 to 415 (107 residues), 58.3 bits, see alignment E=2.4e-19

Best Hits

KEGG orthology group: K12542, membrane fusion protein LapC (inferred from 100% identity to ppf:Pput_0185)

Predicted SEED Role

"Type I secretion system, membrane fusion protein LapC"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RG4 at UniProt or InterPro

Protein Sequence (458 amino acids)

>PP_0166 HlyD family secretion protein (Pseudomonas putida KT2440)
MLHKLDMGQFKDGLRRYFKGSDSLGGQPLPEVNKALIEDAPRVVRLTIWGVILFFVFLIV
WASVAPIDEVTRGEGKAIPSSKVQKIQNLEGGIVAEIFAKEGQIVEVGQPLLRLDETRFA
SNVGETEADRLAMALRVERLSAEVEDRPLIIDEKLRKAAPNQAASEESLYQSRRQQLQDE
IGGLQQQLVQRQQELREYSSKRTQYANSLELLRKEISMSEPLVATGAISQVEVLRLRRAE
VENRGQLDSTALAIPRAEAAIREVQSKIEETRGKFRSEALTQLNEARTELNKATATSKAL
DDRVHRTLVTSPVRGIVKQLLVNTIGGVIQPGSDIIEVVPLDDTLVIEAKILPKDIAFLH
PGQEATVKFTAYDYTIYGGLKAKLEQIGADTITDEDKKTTYYLIKLRTDRSHLGTDEKPL
LIIPGMVATVDIMTGKKTIMSYLLKPIMKARSEALRER