Protein Info for PP_0158 in Pseudomonas putida KT2440

Annotation: glutaryl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02771: Acyl-CoA_dh_N
  • amino acids 20 to 132 (113 residues), 133.6 bits, E=8.4e-43
• PF02770: Acyl-CoA_dh_M
  • amino acids 136 to 227 (92 residues), 78.4 bits, E=7.8e-26
• PF00441: Acyl-CoA_dh_1
  • amino acids 240 to 385 (146 residues), 101.5 bits, E=1e-32
• PF08028: Acyl-CoA_dh_2
  • amino acids 254 to 374 (121 residues), 31.3 bits, E=4.6e-11

Best Hits

Swiss-Prot: 66% identical to GCDH_BOVIN: Glutaryl-CoA dehydrogenase, mitochondrial (GCDH) from Bos taurus

KEGG orthology group: K00252, glutaryl-CoA dehydrogenase [EC: 1.3.99.7] (inferred from 99% identity to ppg:PputGB1_0175)

MetaCyc: 100% identical to glutaryl-CoA dehydrogenase (Pseudomonas putida KT2440)
GLUTARYL-COA-DEHYDROGENASE-RXN [EC: 1.3.8.6]

Predicted SEED Role

"Glutaryl-CoA dehydrogenase (EC 1.3.99.7)" (EC 1.3.99.7)

MetaCyc Pathways

• glutaryl-CoA degradation (3/5 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (4/16 steps found)
• L-tryptophan degradation III (eukaryotic) (3/15 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (4/17 steps found)
• toluene degradation VI (anaerobic) (4/18 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Fatty acid metabolism
• Lysine degradation
• Tryptophan metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.8.6 or 1.3.99.7

Sequence Analysis Tools

See Q88RH2 at UniProt or InterPro

Protein Sequence (393 amino acids)

>PP_0158 glutaryl-CoA dehydrogenase (Pseudomonas putida KT2440)
MVGKASFNWIDPLLLDQQLTEEERMVRDSAYQFAQDKLAPRVLEAFRHEQTDPAIFREMG
EVGLLGATIPEQYGGSGLNYVCYGLIAREVERIDSGYRSMMSVQSSLVMVPINEFGTEAQ
KQKYLPKLASGEWIGCFGLTEPNHGSDPGSMITRARKVDGGYRLTGSKMWITNSPIADVF
VVWAKDDAGDIRGFVLEKGWQGLSAPAIHGKVGLRASITGEIVMDNVFVPEENIFPDVRG
LKGPFTCLNSARYGISWGALGAAEACWHTARQYTLDRQQFGRPLAANQLIQKKLADMQTE
ITLALQGCLRLGRMKDEGTAAVEITSIMKRNSCGKALDIARMARDMLGGNGISDEFGVAR
HLVNLEVVNTYEGTHDVHALILGRAQTGIQAFY