Protein Info for PP_0145 in Pseudomonas putida KT2440

Annotation: Na+/Pi-cotransporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 552 transmembrane" amino acids 6 to 25 (20 residues), see Phobius details amino acids 46 to 69 (24 residues), see Phobius details amino acids 71 to 72 (2 residues), see Phobius details amino acids 75 to 97 (23 residues), see Phobius details amino acids 103 to 121 (19 residues), see Phobius details amino acids 133 to 154 (22 residues), see Phobius details amino acids 172 to 198 (27 residues), see Phobius details amino acids 204 to 226 (23 residues), see Phobius details amino acids 238 to 262 (25 residues), see Phobius details amino acids 274 to 293 (20 residues), see Phobius details TIGR00704: Na/Pi-cotransporter II-related protein" amino acids 1 to 308 (308 residues), 348.6 bits, see alignment E=4e-108 PF02690: Na_Pi_cotrans" amino acids 13 to 147 (135 residues), 121.2 bits, see alignment E=3.3e-39 amino acids 159 to 245 (87 residues), 34.5 bits, see alignment E=2e-12 TIGR01013: sodium-dependent inorganic phosphate (Pi) transporter" amino acids 67 to 521 (455 residues), 346.9 bits, see alignment E=1.9e-107

Best Hits

Swiss-Prot: 64% identical to YJBB_ECO57: Uncharacterized protein YjbB (yjbB) from Escherichia coli O157:H7

KEGG orthology group: K03324, phosphate:Na+ symporter (inferred from 100% identity to ppu:PP_0145)

MetaCyc: 64% identical to putative inorganic phosphate export protein YjbB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-470

Predicted SEED Role

"Sodium-dependent phosphate transporter" in subsystem Phosphate metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RI4 at UniProt or InterPro

Protein Sequence (552 amino acids)

>PP_0145 Na+/Pi-cotransporter (Pseudomonas putida KT2440)
MLTLLNLLSAVTLLVWGTHIVRTGILRVFGSNLRRIISQNMDKRPLAFIAGILVTAMVQS
SNATAMLVTSFVGQGLMAMTPALAIMLGADVGTALMARVLTFDLSWLSPLLIFLGVIFFL
SRKQTRAGQLGRVGIGLGLIILALELIVQAAAPITQAQGVKVLFASLTGDIMLDALVGAM
FAMISYSSLAAVLLTATLAGAELISLPVAIGLVVGANIGSGLLAFISTSMQNAAGRRVAL
GSLLYKLIGLVLIIPVLHPLVAWMDSLSFSPQELVIGFHLLYNTLRCLIMLPTVKPMGRL
CNALLPEREIGNGQVRPRHLDASALETPSLALANAARETLRLGDIVDSLLEAMLGALRGT
QTALPQQVRALGEDAEALYSAIKLYLAQMSREDLSEQDNRRWAEIIELSINLKLACDLIE
RMLRKVQQQKTSQRREFSQVGLEELTGLQEQLLANLRLGLSVFLSADPESARLLLREKRR
FRAQERRLAHAHVSRLQRKVVQSIETSSLHLELIADMKRLNSLFCSSAYVVLGGSDTGGL
MLDSVPDEARLP