Protein Info for PP_0140 in Pseudomonas putida KT2440

Annotation: conserved protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 312 transmembrane" amino acids 7 to 29 (23 residues), see Phobius details PF02470: MlaD" amino acids 39 to 116 (78 residues), 59.8 bits, see alignment E=1.3e-20

Best Hits

KEGG orthology group: K02067, putative ABC transport system substrate-binding protein (inferred from 100% identity to ppu:PP_0140)

Predicted SEED Role

"ABC-type transport system involved in resistance to organic solvents, periplasmic component USSDB6C"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RI9 at UniProt or InterPro

Protein Sequence (312 amino acids)

>PP_0140 conserved protein of unknown function (Pseudomonas putida KT2440)
METRAHHVLIGLVTVLVVTGAMLFGLWLAKSSVDDAFKDYEVVFNEAVSGLSPGSTVQYN
GIKVGDVSTLRLDPKDPRRVLARVRLSADTPVKEDTQAKLTLAGVTGNSFIQLSGGTPQS
PELKGKDGKLPVIIASPSPISRLLNDSSDLVTNINMLLHNANQMFSEDNIGHLSNTLANL
DKTTGAFAGQHGSIAEAIAQLVQVGKQASATLAETQALMRNANGLLDSEGKQAIGSAEQA
MQSLAQSTATINNLLQNNSEAIGDGAQSLNQLAPAIRELRETLNALKGISRQLEADPSGY
LLGRDNNKEFQP