Protein Info for PP_0132 in Pseudomonas putida KT2440

Annotation: Alginate biosynthesis sensor protein KinB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 593 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 166 to 188 (23 residues), see Phobius details PF16767: KinB_sensor" amino acids 41 to 156 (116 residues), 140.7 bits, see alignment E=1.2e-44 PF00672: HAMP" amino acids 191 to 241 (51 residues), 42 bits, see alignment 3.3e-14 TIGR00229: PAS domain S-box protein" amino acids 255 to 371 (117 residues), 35.3 bits, see alignment E=5.4e-13 PF13188: PAS_8" amino acids 256 to 309 (54 residues), 30.3 bits, see alignment 9.9e-11 PF00989: PAS" amino acids 257 to 364 (108 residues), 37.7 bits, see alignment E=6.5e-13 PF08448: PAS_4" amino acids 262 to 369 (108 residues), 51.3 bits, see alignment E=4.5e-17 PF00512: HisKA" amino acids 374 to 441 (68 residues), 57.1 bits, see alignment E=5.4e-19 PF02518: HATPase_c" amino acids 488 to 592 (105 residues), 99.1 bits, see alignment E=7.3e-32

Best Hits

KEGG orthology group: K11383, two-component system, NtrC family, sensor histidine kinase KinB [EC: 2.7.13.3] (inferred from 100% identity to ppu:PP_0132)

Predicted SEED Role

"Sensory box histidine kinase"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RJ7 at UniProt or InterPro

Protein Sequence (593 amino acids)

>PP_0132 Alginate biosynthesis sensor protein KinB (Pseudomonas putida KT2440)
MKWPPMKLRTRLFLSISTLVTVALLGLLLGLVSMLQMATVQQRLVRDTTHALEVGLKLRQ
NLGEQLMLILDEDTAPESLRLLQDNFQDLLNQGLEQGGERTGFSKASSNYQAFLQTYRDS
ATPARSMGVDQPLGAAFNQVRTDLIDSHKQALDLITRSEERTRDRALLVSGVLGLMGLVV
LVLGFITAHNIARRFGQPIEALAKAADQLGKGNFDVTLPVTQAAELNQLTRRFGLMADAL
RKHQATNVDELLAGQQRLQAVLDSIDDGLLIIDRQGRLEHLNPVAQRQLGWNDSRLGSSL
VEALQRPELEQQLRQVLRGGSLDRPPDDLNMEVDEESRLLTYSLTPVSHPQGPILGAVMV
LHDVTEQRAFERVRSEFVLRASHELRTPVTGMHMAFGLLRERVKFPPEARENDLLETIGE
EMQRLTQLINDLLNFSRYQSGLQKLELAPCAIDDLLDHAQSRFAEQAAHKQIELIKELDP
PLPRIQADVAQLDRVLDNLLHNAIRHTANGGRIRLHARRHAERVIISVEDNGEGISYGQQ
ARIFEPFVQVGRKKGGAGLGLALCKEIVQLHGGRMGVFSRPGQGTQFYMALPV