Protein Info for PP_0131 in Pseudomonas putida KT2440

Annotation: putative membrane-anchored phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 535 signal peptide" amino acids 1 to 42 (42 residues), see Phobius details transmembrane" amino acids 252 to 275 (24 residues), see Phobius details PF12792: CSS-motif" amino acids 49 to 248 (200 residues), 130.7 bits, see alignment E=5.2e-42 PF00563: EAL" amino acids 284 to 516 (233 residues), 211.4 bits, see alignment E=1.3e-66

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_0131)

Predicted SEED Role

"Rtn protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RJ8 at UniProt or InterPro

Protein Sequence (535 amino acids)

>PP_0131 putative membrane-anchored phosphodiesterase (Pseudomonas putida KT2440)
MPSMSAFIALLRQIFYRPWMLATLAALASAAVLLSASIGIALQQMKQSESEQMNAQGERF
LERLEQVFGQLREGVDLLQAQPLRGCSPAMLAALQQVGLNSRFIYEAAYVDGNVACSNRG
DERAFVPLRAPDIQGPTYSYWLNTTTEPNENLAALMLGRGKFLVSTSRGHLTDVVDLPPG
GSLVVVLDNGARAIPVLGPPQVWPPPSAWSTSHKSLLELSDRLIYRMPTKSPDYQLVLIA
PRASLPLKMNGMLWLLFPGSVLAACCIGWLVLQLILQRRSMSSELQNALRRGELQVLYQP
IIELDSRRCVGAEALVRWRRPDGTLTSPDLFIPLAENTGQIRQITDFVLQRVLEQLGQLL
RSHPKLYISINLAACDVMVPRIGRVAARLLALHRVAPSQIAFEVTERGLIDVVVARDNLQ
ALRAVGHQVLIDDFGTGYCSLAYLQTLPVDCLKIDKAFIDALGHDAASSGVAPHIIRMAH
DLHLRVIAEGIEFEDQAVLLNSEGVNYGQGWLFARPLNARQFAELVTRGHLPRRV