Protein Info for PP_0117 in Pseudomonas putida KT2440

Annotation: Zn(2+) ABC transporter - permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 260 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details transmembrane" amino acids 18 to 26 (9 residues), see Phobius details amino acids 40 to 73 (34 residues), see Phobius details amino acids 84 to 105 (22 residues), see Phobius details amino acids 119 to 148 (30 residues), see Phobius details amino acids 168 to 202 (35 residues), see Phobius details amino acids 214 to 233 (20 residues), see Phobius details amino acids 239 to 257 (19 residues), see Phobius details PF00950: ABC-3" amino acids 2 to 256 (255 residues), 252.1 bits, see alignment E=3.2e-79

Best Hits

Swiss-Prot: 59% identical to ZNUB_ECOLI: High-affinity zinc uptake system membrane protein ZnuB (znuB) from Escherichia coli (strain K12)

KEGG orthology group: K09816, zinc transport system permease protein (inferred from 99% identity to ppf:Pput_0134)

MetaCyc: 59% identical to Zn2+ ABC transporter membrane subunit (Escherichia coli K-12 substr. MG1655)
ABC-63-RXN [EC: 7.2.2.20]

Predicted SEED Role

"Zinc ABC transporter, inner membrane permease protein ZnuB" in subsystem Transport of Zinc

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.2.2.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RL2 at UniProt or InterPro

Protein Sequence (260 amino acids)

>PP_0117 Zn(2+) ABC transporter - permease subunit (Pseudomonas putida KT2440)
MADFLLYALLAGLSLALVAGPLGSFVVWRRMAYFGDTLSHAALLGVALGFALDVSPALAV
TVGCLLLAILLVTLQQRQPLASDTLLGILAPSTLSLGLVVLSFMHDVRIDLMAYLFGDLL
AISTTDLAWILGGSALVLLLLAALWRPLLAVTVHEELAMVEGLPVAGLRLALMLLIAVVI
AVAMKIVGVLLITSLLIIPAAAAQRHARSPEQMALGASLMGVTAVCGGLAMSWFKDTPAG
PSIVVCAAVLFLLSLALPKR