Protein Info for PP_0110 in Pseudomonas putida KT2440

Annotation: CyoE like protoheme IX farnesyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 transmembrane" amino acids 21 to 39 (19 residues), see Phobius details amino acids 47 to 69 (23 residues), see Phobius details amino acids 90 to 113 (24 residues), see Phobius details amino acids 119 to 137 (19 residues), see Phobius details amino acids 145 to 165 (21 residues), see Phobius details amino acids 172 to 191 (20 residues), see Phobius details amino acids 216 to 235 (20 residues), see Phobius details amino acids 241 to 261 (21 residues), see Phobius details amino acids 273 to 294 (22 residues), see Phobius details TIGR01473: protoheme IX farnesyltransferase" amino acids 13 to 291 (279 residues), 348 bits, see alignment E=2.7e-108 PF01040: UbiA" amino acids 28 to 273 (246 residues), 229.8 bits, see alignment E=1.7e-72

Best Hits

Swiss-Prot: 100% identical to CYOE1_PSEPK: Protoheme IX farnesyltransferase 1 (cyoE1) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K02301, protoheme IX farnesyltransferase [EC: 2.5.1.-] (inferred from 100% identity to ppu:PP_0110)

MetaCyc: 33% identical to heme O synthase (Escherichia coli K-12 substr. MG1655)
HEMEOSYN-RXN [EC: 2.5.1.141]

Predicted SEED Role

"Cytochrome oxidase biogenesis protein Sco1/SenC/PrrC, putative copper metallochaperone" in subsystem Biogenesis of cytochrome c oxidases

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.-

Use Curated BLAST to search for 2.5.1.- or 2.5.1.141

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RL9 at UniProt or InterPro

Protein Sequence (297 amino acids)

>PP_0110 CyoE like protoheme IX farnesyltransferase (Pseudomonas putida KT2440)
MATLLSVRRARWRDYLELTKPKVVVLMLITSLAGMFLATRAGVSWSVLLFGNLGIGLCAG
GAAVVNHVVDRRIDALMARTHKRPLAQGRVEPLPALLFALALALLGMVLLLVFTNALTAW
LTLASLLGYAVLYTGFLKRATPQNIVIGGLAGAAPPLLGWVAVSGHVSAEPLLLVLIIFA
WTPPHFWALAIHRKEEYAKADIPMLPVTHGERYTKLHILLYTLILLAVSLLPYAIHMSGP
LYLVCALALGLRFLQWAWVLYRSSRPHAAIGTFKYSIGYLFALFIALLLDHYLLLNL