Protein Info for PP_0067 in Pseudomonas putida KT2440

Annotation: Methionyl-tRNA formyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 310 TIGR00460: methionyl-tRNA formyltransferase" amino acids 1 to 302 (302 residues), 320.2 bits, see alignment E=6.6e-100 PF00551: Formyl_trans_N" amino acids 1 to 180 (180 residues), 164.6 bits, see alignment E=2.2e-52 PF02911: Formyl_trans_C" amino acids 203 to 298 (96 residues), 99.3 bits, see alignment E=1.2e-32

Best Hits

Swiss-Prot: 100% identical to FMT_PSEPK: Methionyl-tRNA formyltransferase (fmt) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00604, methionyl-tRNA formyltransferase [EC: 2.1.2.9] (inferred from 100% identity to ppu:PP_0067)

MetaCyc: 59% identical to 10-formyltetrahydrofolate:L-methionyl-tRNAfMet N-formyltransferase (Escherichia coli K-12 substr. MG1655)
Methionyl-tRNA formyltransferase. [EC: 2.1.2.9]; 2.1.2.9 [EC: 2.1.2.9]

Predicted SEED Role

"Methionyl-tRNA formyltransferase (EC 2.1.2.9)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or Folate Biosynthesis (EC 2.1.2.9)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RR2 at UniProt or InterPro

Protein Sequence (310 amino acids)

>PP_0067 Methionyl-tRNA formyltransferase (Pseudomonas putida KT2440)
MRIVFAGTPEFAAEHLKALLDSPYEIVAVYTQPDRPAGRGQKLMPSAVKALAVAHDIPVF
QPQTLRNADAQAELAALKPDLMVVVAYGLILPQVVLDIPRLGCINSHASLLPRWRGAAPI
QRAVEAGDAESGVTVMRMEAGLDTGPMLLKVVTPISAEDTGGTLHDRLAEMGPPAVVQAI
AGLADGSLQGEIQDDALATYAHKLNKDEARIDWTHPAVELERLIRAFNPWPVCHSTLDGE
SVKVLAANLSTGQGAPGEILSASKDGLVVACGDQALSLTRLQLPGGKALSFSDLFNSRRE
KFAAGKVLGQ