Protein Info for PP_0029 in Pseudomonas putida KT2440

Annotation: two component heavy metal response regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 224 TIGR01387: heavy metal response regulator" amino acids 3 to 220 (218 residues), 342.2 bits, see alignment E=6.2e-107 PF00072: Response_reg" amino acids 3 to 112 (110 residues), 98.8 bits, see alignment E=2.1e-32 PF00486: Trans_reg_C" amino acids 145 to 220 (76 residues), 93.5 bits, see alignment E=6.8e-31

Best Hits

Swiss-Prot: 60% identical to CZCR_CUPMC: Transcriptional activator protein CzcR (czcR) from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

KEGG orthology group: None (inferred from 100% identity to ppf:Pput_0043)

Predicted SEED Role

"DNA-binding heavy metal response regulator" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RV0 at UniProt or InterPro

Protein Sequence (224 amino acids)

>PP_0029 two component heavy metal response regulator (Pseudomonas putida KT2440)
MRVLVVEDEIKTAEYLQQGLSESGYVVDIVHNGVDALHLFNTNVYSLVLLDVNLPGIDGW
DLLETIRKTSRVRIIMLTARGRINDKLKGLDGGADDYLVKPFEFPELLARIRSLQRRGDE
LVEKSSLKIADLELDSVRHRVFRGGTRIDLTTKEFALLHLLMSRTGEALTRSQIISLVWD
MNFDCDTNVIDVAIRRLRSKIDDPFETKLIHTLRGVGYVFEERA