Protein Info for PP_0004 in Pseudomonas putida KT2440

Annotation: tRNA uridine 5-carboxymethylaminomethyl modification enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 631 TIGR00136: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA" amino acids 8 to 621 (614 residues), 921.7 bits, see alignment E=9.5e-282 PF12831: FAD_oxidored" amino acids 9 to 143 (135 residues), 39.8 bits, see alignment E=9.1e-14 PF01134: GIDA" amino acids 9 to 399 (391 residues), 579.1 bits, see alignment E=1.2e-177 PF21680: GIDA_C_1st" amino acids 461 to 555 (95 residues), 96.9 bits, see alignment E=2.4e-31 PF13932: SAM_GIDA_C" amino acids 560 to 615 (56 residues), 84.3 bits, see alignment 1e-27

Best Hits

Swiss-Prot: 100% identical to MNMG_PSEPK: tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03495, glucose inhibited division protein A (inferred from 100% identity to ppu:PP_0004)

MetaCyc: 69% identical to 5-carboxymethylaminomethyluridine-tRNA synthase subunit MnmG (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88RW8 at UniProt or InterPro

Protein Sequence (631 amino acids)

>PP_0004 tRNA uridine 5-carboxymethylaminomethyl modification enzyme (Pseudomonas putida KT2440)
MVDFPSRFDVIVIGGGHAGTEAALASARMGVKTLLLTHNVETLGHMSCNPAIGGIGKSHL
VKEIDALGGAMALATDKSGIQFRVLNNRKGPAVRATRAQADRAIYKAVVREILENQPNLW
IFQQSCDDLIVEQDQVKGVVTQMGLRFFAESVVLTTGTFLGGLIHIGLQNHSGGRAGDPP
SIALAHRMRELPLRVGRLKTGTPPRIDGRSVDFSVMTEQPGDTPIPVMSFMGNAEMHPRQ
VSCWITHTNARTHEIIASNLDRSPMYSGVIEGVGPRYCPSIEDKIHRFADKESHQVFIEP
EGLNTHELYPNGISTSLPFDVQLELVRSIRGMENAHIVRPGYAIEYDYFDPRDLKYSLET
KVIGGLFFAGQINGTTGYEEAGAQGLLAGTNAALRAQGRDSWCPRRDEAYIGVLVDDLIT
LGTQEPYRMFTSRAEYRLILREDNADLRLTEKGRELGLIDDQRWAAFCAKRDGIEREEQR
LKSTWVRPNTEQGQAIVDKFGTPLSHEYSLLNLLARPEIDYAGLIEATGGEAIDPQVAEQ
VEIRTKYAGYIDRQQDEIARLRASEDTRLPVDIDYTTISGLSKEIQGKLSQTRPQTLGQA
SRIPGVTPAAISLLLIHLKKRGAGRELEQSA