Protein Info for PFR28_04587 in Pseudomonas sp. RS175

Annotation: tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 630 TIGR00136: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA" amino acids 7 to 620 (614 residues), 918.8 bits, see alignment E=7.3e-281 PF01134: GIDA" amino acids 8 to 398 (391 residues), 578.7 bits, see alignment E=1.6e-177 PF12831: FAD_oxidored" amino acids 8 to 150 (143 residues), 35.4 bits, see alignment E=2.1e-12 PF21680: GIDA_C_1st" amino acids 460 to 554 (95 residues), 93.4 bits, see alignment E=2.9e-30 PF13932: GIDA_C" amino acids 559 to 614 (56 residues), 82.2 bits, see alignment 4.5e-27

Best Hits

Swiss-Prot: 97% identical to MNMG_PSEF5: tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K03495, glucose inhibited division protein A (inferred from 97% identity to pfl:PFL_6226)

MetaCyc: 69% identical to 5-carboxymethylaminomethyluridine-tRNA synthase subunit MnmG (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (630 amino acids)

>PFR28_04587 tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (Pseudomonas sp. RS175)
MDFPSRFEVIVIGGGHAGTEAALASARMGVKTLLLTHNVETLGAMSCNPAIGGIGKSHLV
KEIDALGGAMAMATDKGGIQFRVLNSRKGPAVRATRAQADRVLYKAAVREILENQPNLWI
FQQAADDLIVEQDQVRGVVTQMGLRFFAESVVLTTGTFLGGLIHIGLQNYSGGRAGDPPS
IALAQRLRELPLRVGRLKTGTPPRIDGRSVDFSVMTEQPGDTPIPVMSFMGSKEQHPKQV
SCWITHTNARTHEIIAANLDRSPMYSGVIEGVGPRYCPSIEDKIHRFADKESHQVFIEPE
GLTTHELYPNGISTSLPFDVQLQIVQSIRGMENAHIVRPGYAIEYDYFDPRDLKYSLETK
VIGGLFFAGQINGTTGYEEAGAQGLLAGTNAALRARGQDSWCPRRDEAYIGVLVDDLITL
GTQEPYRMFTSRAEYRLILREDNADLRLTEKGRELGLVDDVRWAAFCKKRESIALEEQRL
KSTWVRPGTEQGDAIAARFGTPLTHEYNLLNLLSRPEIDYAGLVEVTGEGAQDPQVAEQV
EIKIKYAGYIDRQQDEIARLRASEDTKLPVDIDYTVISGLSKEIQSKLGATRPETLGQAS
RIPGVTPAAISLLMIHLKKRGAGRQLEQSA