Protein Info for PFR28_03732 in Pseudomonas sp. RS175

Annotation: Type II secretion system protein F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 405 transmembrane" amino acids 171 to 192 (22 residues), see Phobius details amino acids 199 to 217 (19 residues), see Phobius details amino acids 224 to 243 (20 residues), see Phobius details amino acids 257 to 273 (17 residues), see Phobius details amino acids 377 to 399 (23 residues), see Phobius details PF00482: T2SSF" amino acids 71 to 194 (124 residues), 120 bits, see alignment E=3.2e-39 amino acids 275 to 397 (123 residues), 112.1 bits, see alignment E=9.1e-37

Best Hits

Swiss-Prot: 57% identical to TAPC_AERHY: Type IV pilus assembly protein TapC (tapC) from Aeromonas hydrophila

KEGG orthology group: K02653, type IV pilus assembly protein PilC (inferred from 93% identity to pba:PSEBR_a4827)

Predicted SEED Role

"Type IV fimbrial assembly protein PilC" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (405 amino acids)

>PFR28_03732 Type II secretion system protein F (Pseudomonas sp. RS175)
MAVKAVKTDTYTWEGKDRKGTKMSGELTGQSPALVKAQLRKQGINPDRVRKKSTSIFSKG
KRIKPLDIALFTRQMATMLKAGVPLLQAFDIIGEGFDNANMRKLIDEVKQEVAAGNSFAA
SLRKCPQYFDELYCNLVDAGEQAGALDTLLDRVATYKEKSEALKAKIKKAMTYPTAVILV
AAVVTGILLVKVVPQFESVFAGFGAELPGFTVMVIGLSEFMQQWWWLLLGALVGGFFGVR
YALKRSQGFRDWRDKWLLKLPLIGALMYKSAVARFARTLSTTFAAGVPLVEALDSVSGAT
GNVVFKRAVQRIRQDVSTGMQLNFSMRASGIFPNLAIQMTAIGEESGALDDMLDKVASFY
EAEVDNLVDNLTSLMEPFIMVVLGVVVGGLVVAMYLPIFQLGSAI