Protein Info for PFR28_02012 in Pseudomonas sp. RS175

Annotation: Multidrug resistance protein MdtA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 388 signal peptide" amino acids 1 to 39 (39 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 48 to 377 (330 residues), 247.9 bits, see alignment E=6.3e-78 PF16576: HlyD_D23" amino acids 72 to 296 (225 residues), 47.1 bits, see alignment E=2.7e-16 PF13533: Biotin_lipoyl_2" amino acids 73 to 120 (48 residues), 33.7 bits, see alignment 3.7e-12 PF13437: HlyD_3" amino acids 184 to 293 (110 residues), 28.3 bits, see alignment E=3.8e-10

Best Hits

KEGG orthology group: None (inferred from 51% identity to bte:BTH_II2106)

Predicted SEED Role

"RND efflux system, membrane fusion protein CmeA" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (388 amino acids)

>PFR28_02012 Multidrug resistance protein MdtA (Pseudomonas sp. RS175)
MSSFIRRLSGVPVWALGLLALALIAGFAISMRAPVAQAKSAPAPLPQVQVMSAITRDVVD
WQSYTGRLVATDTVVIKPLVAGTLISVHFKDGALLRKGDLMFEIDPRPYAATLQQAQAAL
VAARSMSLFAQKELQRYEHLITSNAIAKTDLDKRIQSANEAQARVNEAQARVELARLDLE
HTRILAPFDGRASRVEVTPGNIVDVGERSTALTNLVSISPIYADFDVDEQTFLRYLGNRP
AGDPVPVMLGLAHEEGYPRKGNLSSLDNRLDTRSATIRVRASFDNSNGELRPGLFARVLL
PGSASRPATLVDDSAIGTDQDKKYVLVVDAEGRVQYRQVKPGIRQGALRVIDQGISPEEQ
VVVSSLHLATPGSQVAPVRAPSQPQAQQ