Protein Info for PFR28_01523 in Pseudomonas sp. RS175

Annotation: Adaptive-response sensory-kinase SasA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 462 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 161 to 181 (21 residues), see Phobius details TIGR01386: heavy metal sensor kinase" amino acids 3 to 452 (450 residues), 407.8 bits, see alignment E=3.3e-126 PF00672: HAMP" amino acids 179 to 229 (51 residues), 33.6 bits, see alignment 5.9e-12 PF00512: HisKA" amino acids 237 to 302 (66 residues), 54.3 bits, see alignment E=1.7e-18 PF02518: HATPase_c" amino acids 346 to 446 (101 residues), 68.3 bits, see alignment E=1.2e-22

Best Hits

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 87% identity to pba:PSEBR_a2894)

Predicted SEED Role

No annotation

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (462 amino acids)

>PFR28_01523 Adaptive-response sensory-kinase SasA (Pseudomonas sp. RS175)
MMKRSIARRLALMFALAAMLITSVSATLLRCSLKQSLNEQMQNELILRHSVLDPVLAKYD
SPAFWVGLRQKLDGLTPADERVRYWVVVDNPAYSYGGQMPDGQDWSKRPEGFSTLEIPDR
ARPMMLLVKTIPALGSRPELRFIVGLDSTPFMKTLGHFTKILILISAVGVVLVALLGYWI
ARFGLGPVRRLSRQANSLPPGDSKQRLDTEALPGELQELAVSFNGALARQEAAWCQLEGF
NANVAHELRTPLTNLIGQTQVALAHGRDVGELQDLLQSNLEELERMATLVNDMLFLAGAE
SGQRATELSDVSLYEEASKTVEYLEPVLLDRQLNVVVTGDMRARIDRRLFHRSLANLLQN
AARYAVPASTITVNLVSQGIQVEVSVSNAGDPIDTVHLEHLFERFYRADAARARSDAHHG
LGLSIVRAVASMHGGRVFAHSKDGFNTFGFSLMSQALPHDSV