Protein Info for PFR28_01074 in Pseudomonas sp. RS175

Annotation: Holliday junction ATP-dependent DNA helicase RuvB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 689 TIGR02397: DNA polymerase III, subunit gamma and tau" amino acids 3 to 355 (353 residues), 436.2 bits, see alignment E=5.1e-135 PF05496: RuvB_N" amino acids 11 to 73 (63 residues), 29.9 bits, see alignment E=1.4e-10 PF13177: DNA_pol3_delta2" amino acids 20 to 178 (159 residues), 150.5 bits, see alignment E=1.3e-47 PF07728: AAA_5" amino acids 42 to 145 (104 residues), 23.1 bits, see alignment E=2e-08 PF00004: AAA" amino acids 42 to 171 (130 residues), 45.1 bits, see alignment E=4.1e-15 PF12169: DNA_pol3_gamma3" amino acids 232 to 362 (131 residues), 107.4 bits, see alignment E=1.7e-34 PF12170: DNA_pol3_tau_5" amino acids 560 to 678 (119 residues), 62.4 bits, see alignment E=1.5e-20

Best Hits

KEGG orthology group: K02343, DNA polymerase III subunit gamma/tau [EC: 2.7.7.7] (inferred from 92% identity to pba:PSEBR_a1712)

Predicted SEED Role

"DNA polymerase III subunits gamma and tau (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (689 amino acids)

>PFR28_01074 Holliday junction ATP-dependent DNA helicase RuvB (Pseudomonas sp. RS175)
MSYQVLARKWRPRSFREMVGQTHVLKALINALDSQRLHHAYLFTGTRGVGKTTIARIIAK
CLNCETGITSTPCGECSVCREIDEGRFVDLIEIDAASRTKVEDTRELLDNVQYAPSRGRF
KVYLIDEVHMLSSHSFNALLKTLEEPPPYVKFILATTDPQKLPATILSRCLQFSLKNMTP
ERVVEHLTHVLGVENVPFEDDALWLLGRAADGSMRDAMSLTDQAIAFGEGKVMAADVRAM
LGTLDHGQVYDVLHALIEGDAKALLEAVRHLAEQGPDWNGVLSEILNVLHRVAIAQALPE
GVDNGHGDRDRVLALAQALPAEDVQFYYQMGLIGRRDLPLAPDPRGGFEMVLLRMLAFRP
ADTADAPRPVLKPVGISQATADSTRPVAAAPVVAPAVVSAPVTVAAIAQPAPTPVVAAAQ
PVPEPEPAPEPVAEAVIDLPWNDPVEPEIVQPPARQPVLDTTAQQPELPPLPMPTPDSVV
PDAPEWVAAPMPEPTAAEVDLATPGTDLDDEPPLDEDYIEPDLDSAYSYLDDLASEHARE
PTPEPEPEPAAMPATGLALQWLELFAKLPISGMTGSIAANCTLISVEGDHWLLHLDPAHS
ALFNATQQRRLNDALNQYHQRTLTLSIELIKPEQETPAQAAARRRADRQREAEESIHNDP
FIQQMMQQFGAVVRHDTIEPVEAPVSQGS