Protein Info for PFR28_00773 in Pseudomonas sp. RS175

Annotation: HTH-type transcriptional regulator GltR

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 transmembrane" amino acids 219 to 236 (18 residues), see Phobius details PF00126: HTH_1" amino acids 4 to 62 (59 residues), 75.4 bits, see alignment E=2.7e-25 PF03466: LysR_substrate" amino acids 85 to 283 (199 residues), 148.5 bits, see alignment E=1.8e-47

Best Hits

Swiss-Prot: 51% identical to YNEJ_ECOLI: Uncharacterized HTH-type transcriptional regulator YneJ (yneJ) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 93% identity to pba:PSEBR_a1422)

Predicted SEED Role

"Transcriptional regulators, LysR family"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (297 amino acids)

>PFR28_00773 HTH-type transcriptional regulator GltR (Pseudomonas sp. RS175)
MEFSQLRIFQAVAEEGSITRAAERMHRVPSNLSTRLRQLEEQLGVDLFRRERQRLQLSPA
GKVLLDYANKLFALHDEAHAAVQGGQPAGDFVLGTMYSTAAIYLPDLLATYHRTYPAVNL
QVQSGPSGELLEGLLTGRLDAALVDGPLELAGLDGVPLCDERLVLISEADHPPVRSALDV
QGRSVFTFRRGCSYRMRLEAWFAHDHATMGRAMEIESYQGMLACVIAGSGVALMSESMLA
SLPGREQVAVHPLAEPFASATTWLMWRKGMVGANLNAWIELQQRAWPPVARREAQSA